Immune thrombocytopenia antiplatelet autoantibodies inhibit proplatelet formation by

Immune thrombocytopenia: antiplatelet autoantibodies inhibit proplatelet formation by megakaryocytes and impair platelet production in vitro by Muna Iraqi, Jose Perdomo, Feng Yan, Philip Y-I Choi, and Beng H. Chong haematol Volume 100(5): 623 -632 May 5, 2015 © 2015 by Ferrata Storti Foundation

Details of ITP patients. Muna Iraqi et al. Haematologica 2015; 100: 623 -632 © 2015 by Ferrata Storti Foundation

Groups of ITP patients based on the effect of the patients’ autoantibodies on the proplatelet formation capacity of MK. (A) Representative histograms of ITP serum binding to platelets, CHOCD 61/CD 41 cells and CHO-CD 42 b/CD 42 a cells. Muna Iraqi et al. Haematologica 2015; 100: 623 -632 © 2015 by Ferrata Storti Foundation

The effect of ITP serum and Ig. G on the number of proplatelet-bearing MK. (A) Compared to the control group, the number of proplatelet-bearing MK was significantly reduced in MK cultures treated with serum and Ig. G from group A samples (P<0. 0001 and P=0. 0012, . . . Muna Iraqi et al. Haematologica 2015; 100: 623 -632 © 2015 by Ferrata Storti Foundation

The effect of ITP serum on platelets released in culture and on total MK numbers and maturation. Muna Iraqi et al. Haematologica 2015; 100: 623 -632 © 2015 by Ferrata Storti Foundation

Effect of ITP serum and Ig. G on MK ploidy distribution and cell size. Muna Iraqi et al. Haematologica 2015; 100: 623 -632 © 2015 by Ferrata Storti Foundation

Romiplostim and eltrombopag treatment reverses the effect of ITP Ig. G on MK proplatelet formation capacity. Muna Iraqi et al. Haematologica 2015; 100: 623 -632 © 2015 by Ferrata Storti Foundation