Identify Patient Zero of a Zombie Apocalypse With

Identify Patient Zero of a Zombie Apocalypse With the Power of an ELISA! Biotechnology Explorer™ | explorer. bio-rad. com

Bio-Rad Curriculum and Training Specialist Damon Tighe damon_tighe@bio-rad. com Tamica Stubbs tamica_stubbs@bio-rad. com Sherri Andrews, Ph. D. sherri_andrews@bio-rad. com Leigh Brown leigh_brown@bio-rad. com Biotechnology Explorer™ | explorer. bio-rad. com

TV or real life? The Walking Dead show Biotechnology Explorer™ | explorer. bio-rad. com Feb 11, 2014 Atlanta

Zombiefication § Method of infection – Bodily fluid contact – Airborne – Foodborne § Symptoms – Undead – Hungry for flesh § Treatment – None known Biotechnology Explorer™ | explorer. bio-rad. com

§ Introduction to Immunology, Antibodies and ELISA § Fluid swapping and disease spreading! § Indirect ELISA to narrow down who were the initial carriers of the disease Biotechnology Explorer™ | explorer. bio-rad. com

Crash Course in Immunology and ELISA Immune Response C. Macrophage A. Pathogen Zombie virus D. Macrophage B. B cells F. T cell E. Macrophage G. B cell J. Antibodies attach to pathogen H. Memory B cells Biotechnology Explorer™ | explorer. bio-rad. com I. Plasma cells Antibodies are ineffective Against Zombie Virus

Crash Course in Immunology and ELISA Antibody Structure ELISA tests are based on immune system antibody molecules Heavy chain Light chain Disulfide bonds Antigens Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Enzyme-Linked Immunosorbant Assay (indirect) Add the purified antigen to all the wells. Incubate for 5 min. Rinse Add serum antibodies (student samples) to the appropriate wells. Incubate for 5 min. Rinse Add the enzyme-linked antibody to all wells. Incubate for 5 min. Rinse Add enzyme substrate to all wells. Incubate for 5 min. Biotechnology Explorer™ | explorer. bio-rad. com

Swapping Fluids and Disease Swap with 1 person at your table (record who) Add your 750 µl together, mix and each take 750 µl back Biotechnology Explorer™ | explorer. bio-rad. com

Swapping Fluids and Disease Swap with 1 person at the closest table (record who) Add your 750 µl together, mix and each take 750 µl back Biotechnology Explorer™ | explorer. bio-rad. com

Swapping Fluids and Disease Swap with 1 person at the another table (record who) Add your 750 µl together, mix and each take 750 µl back Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Label Tubes Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Add 50 µl “+” (Antigen) Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

Add 50 ul “-” (wash buffer) Your ID ID # # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

Add 50 ul Bodily Fluid Your ID ID # # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Incubate: 5 minutes Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Protein (Antigen) H R Hydrophobic interactions Polystyrene (plate) Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Tap out liquid Y o u r I D # You ID # r Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Wash with Buffer (contains Tween 20) Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Tap out liquid Y o u r I D # You ID # r Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Wash with Buffer (contains Tween 20) Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Tap out liquid Y o u r I D # You ID # r Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Add 50 µl “PA” (Primary Antibody) Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Incubate: 5 minutes Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Variable region Heavy chain Light chain constant region 3, 368, 600 antibody combos Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Tap out liquid You ID # r Yo u r I D # Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Wash with Buffer Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Tap out liquid You ID # r Yo u r I D # Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Wash with Buffer Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Tap out liquid You ID # r Yo u r I D # Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Add 50 ul “SA” (Secondary Antibody) Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Incubate: 5 minutes Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Amplification of signal Introduce HRP (Horseradish Peroxidase) for colormetric detection Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Antigen = chicken gamma globulin Primary Antibody = anti-chicken polyclonal antibody Secondary Antibody = goat anti-rabbit conjugate to HRP Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Tap out liquid You ID # r Yo u r I D # Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Wash with Buffer Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Tap out liquid You ID # r Yo u r I D # Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Wash with Buffer Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Tap out liquid You ID # r Yo u r I D # Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Wash with Buffer Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Tap out liquid You ID # r Yo u r I D # Biotechnology Explorer™ | explorer. bio-rad. com

ELISA Time! Testing for who is infected Add 50 µl “Sub” (HRP substrate) Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Incubate: 5 minutes Watch for color change! Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Your ID # Biotechnology Explorer™ | explorer. bio-rad. com Your ID #

ELISA Time! Testing for who is infected Concept Corner Biotechnology Explorer™ | explorer. bio-rad. com

Deducing who was originally infected Biotechnology Explorer™ | explorer. bio-rad. com

Deducing who was originally infected Everyone Stand UP! If your ELISA was negative sit down Biotechnology Explorer™ | explorer. bio-rad. com

Deducing who was originally infected § Round 1 – If someone you shared with just sat down, also sit down § Round 2 – If someone you shared with just sat down, also sit down § Round 3 – If someone you shared with just sat down, also sit down Biotechnology Explorer™ | explorer. bio-rad. com

Deducing who was originally infected Who is left? Biotechnology Explorer™ | explorer. bio-rad. com

What did we use today? Bio-Rad’s Immuno Explorer Lab 166 -2400 EDU Biotechnology Explorer™ | explorer. bio-rad. com

Other Zombie themed learning -> § Texas instruments has a program for modeling (via graphing) the spread of a Zombie Virus: § http: //education. ti. com/en/us/stem-hollywood/zombies § Bio-Rad’s Secrets of the Rainforest Kit can be used in a Zombie apocalypse story where students use column chromatography to isolate the Zombie Green Fluorescent Protein. § https: //www. dropbox. com/sh/zbjkffhfsmmpurw/AAAif. Y 6 D 85 Yq. Rw. Wo. BEGe__LGa? dl=0 Biotechnology Explorer™ | explorer. bio-rad. com