Human Leukocyte Antigen HLA Human Major Histocompatibility Complex
Human Leukocyte Antigen (HLA) • Human Major Histocompatibility Complex (MHC) 6 p 21. 3
HLA typing 1. 혈청학적 검사 – 2. 미세림프구 독성검사(microlymphocytotoxicity test) 분자생물학적 검사 – – – Reverse SSO(sequence specific oligonucleotide) hybridization Sequence specific primer method Direct sequencing 3. 세포학적 검사 • • 혼합 림프구 배양검사( one way, two way) HTC (homozygous typing cell) typing
Mononuclear cell의 분리
8 (strong positive, 81 -100%) 6 (positive, 51 -80%) 1 (negative, 1 -10%) 4 (weak positive, 21 -50%)
Microlymphocytotoxicity test
Sequence specific oligonucleotide hybridization method • PCR • Hybridization
Sequence specific primer method
ELISA for HIV antibody Microplate ELISA : colored wells indicate reactivity
Western Blot HIV-1 Western Blot • • • Lane 1: Positive Control Lane 2: Negative Control Sample A: Negative Sample B: Indeterminate Sample C: Positive • 항 p 24, 항 gp 41 또는 gp 120/160 중 2개 이 상이면 양성
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