http www afftis or jpindex html MARIMO Quorum

物語 社団法人　農林水産技術情報協会 http: //www. afftis. or. jp/index. html MARIMO

Quorum Sensing methionine Sender met. K Lux. I S-adenosyl methionine p. Tet Lux. I AHL 3 OC 6 HSL(AHL) Receiver Lux R Lux. R p. Tet GFP p. Lux

AHL quencher ～aii. A～ Sender Lux. I p. Tet AHL aii. A Lacton circle P cons.

aii. A　inverter Receiver Lux. R p. Tet GFP p. Lux Low AHL conc. aii. A c. I inverter High AHL conc.

Senderの数とGFP発現 Sender Receiver Lux. R Lux. I p. Tet GFP p. Lux Sender(BW 25113 or XL 10 G), Methionine (10 m. M or 0 m. M) Receiver Sender: 5× 10^8～ 5× 10

Senderの数とGFP発現 methionine met. K Sender(BW 25113 or XL 10 G), Methionine (10 m. M or 0 m. M) S-adenosyl methionine Lux. I Receiver Sender: 5× 10^8～ 5× 10 3 OC 6 HSL(AHL) Methionine 0 m. M Methionine 10 m. M BW 25113 5× 10^4 XL 10 G 5× 10^7

AHL synthesis methionine S-adenosyl methionine met. K 3 OC 6 HSL Lux. I, acyl-ACP Sender(BW 25113 or XL 10 G), Methionine (10 m. M or 0 m. M) Receiver Sender: 5× 10^8～ 5× 10

sensitive receiver

sensitive receiver (we made)

sensitive receiver (we made)

sensitive receiver (we made)

Flagella Flagellin, Fli. C Flagellum Graduate School of Frontier Biosciences, Osaka University ナノ生体科学講座　プロトニックナノマシン研究室（難波研究室） http: //www. fbs. osaka-u. ac. jp/labo/09 a. html

Flagellin, Fli. C Salmonella typhimurium dispensable indispensable プロトニックナノマシンプロジェクト, ERATO 研究成果 (1) http: //www. npn. jst. go. jp/index_jp. html

Fli. C-His His-Tag

Beads adsorption Beads ・Four of cobalt’s six coordination sites are occupied. ・The imidazole rings of histidine residues are able to occupy the two remaining coordinate sites, resulting in protein binding.

Beads Adsorption

Toxicity-Check Ni 2+ Sender or Receiveｒ Co 2+ : 1μM-!? 100μM-!? 1 m. M-!? Ni 2+ : 1μM-!? 100μM-!? 1 m. M-!?

Sender AHL ＋ Co 2+ aggregated

Receiver aggregation Sender AHL ＋ Co 2+ aggregated

le o az d RR R R S SS i m i + Senderとreceiver ばらばらのコロニーができる S Senderとreceiverを それぞれ培養し、 receiverにsenderを１ 滴加える Senderとreceiver くっついたコロニーができる

顕微鏡写真 +imidazol 顕微鏡写真 Non imidazol

S S S Receiver O/N Culture R R R Co 2+ R R

S S S Receiver O/N Culture R R R Co２＋ R R AHL R R

S S S Receiver O/N Culture R R R S R R AHL Co 2+ S S R R

S S S Receiver O/N Culture R R SR S S R R R AHL Co２＋ R

S S S Receiver O/N Culture R R SR S S R R R AHL Co 2+ R

S S S Receiver O/N Culture R R SR S S R R MARIMO! R AHL Co 2+ R