http mgl scripps edupeoplegoodsell FGF10 FGF8fibroblast growth factor8

細胞の図 http: //mgl. scripps. edu/people/goodsell/

側板中胚葉からFGF-10 FGF-8（fibroblast growth factor-8, chick） 10 20 30 40 50 60 1 MCKWILTNGA SAFSHLPCCC LLLLFLVSSV PVTCHDLGQD MLSPEATNSS SSSSSSFPSS 61 FSSPSSAGRH VRSYNHLQGD VRKRKLYSYN KYFLKIEKNG KVSGTKKENC PFSILEITSV 121 EIGVVAVKSI KSNYYLAMNK KGKVYGSKEF NSDCKLKERI EENGYNTYAS LNWKHNGRQM 181 FVALNGRGAT KRGQKTRRKN TSAHFLPMVV MS FGF（fibroblast growth factor 、繊維芽細 胞増殖因子）は、血管系細胞の増殖や 神経細胞の成長を促すペプチド群の総 称で、いくつかの種類（18種類）がある。

FGF-8もペプチド FGF-8（fibroblast growth factor-8, chick） 10 20 30 40 50 60 1 MDPCSSLFSY VFMHLFVLCL QAQVTVQSPP NFTQHVREQS LVTDQLSRRL VRTYQLYSRT 61 SGKHVQILDN KKINAMAEDG DVHAKLIVET DTFGSRVRIK GAATGFYICM NKKGKLIGKS 121 NGKGKDCVFT EIVLENNYTA LQNAKYEGWY MAFTRKGRPR KGSKTRQHQR EVHFMKRLPK 181 GHQTTEPHRR FEFLNYPFNR RSKRTRNSSA SLRP

FGFは受容体と結合 FGF受容体（chick） 10 20 30 40 50 60 1 MFTWRCLILW AVLVTATLSA ARPAPTLPDQ ALPKANIEVE SHSAHPGDLL QLRCRLRDDV 61 QSINWVRDGV QLPENNRTRI TGEEVEVRDA VPEDSGLYAC MTNSPSGSET TYFSVNVSDA 121 LPSAEDDDDE DDSSSEEKEA DNTKPNQAVA PYWTYPEKME KKLHAVPAAK TVKFKCPSGG 181 TPNPTLRWLK NGKEFKPDHR IGGYKVRYAT WSIIMDSVVP SDKGNYTCIV ENKYGSINHT 241 YQLDVVERSP HRPILQAGLP ANKTVALGSN VEFVCKVYSD PQPHIQWLKH IEVNGSKIGP 301 DNLPYVQILK TAGVNTTDKE MEVLHLRNVS FEDAGEYTCL AGNSIGISHH SAWLTVLEAT 361 EQSPAMMTSP LYLEIIIYCT GAFLISCMVV TVIIYKMKST TKKTDFNSQL AVHKLAKSIP 421 LRRQVTVSAD SSSSMNSGVM LVRPSRLSSS GTPMLAGVSE YELPEDPRWE LPRDRLILGK 481 PLGEGCFGQV VLAEAIGLDK DKPNRVTKVA VKMLKSDATE KDLSDLISEM EMMKMIGKHK 541 NIINLLGACT QDGPLYVIVE YASKGNLREY LQARRPPGME YCYNPTRIPE EQLSFKDLVS 601 CAYQVARGME YLASKKCIHR DLAARNVLVT EDNVMKIADF GLARDIHHID YYKKTTNGRL 661 PVKWMAPEAL FDRIYTHQSD VWSFGVLLWE IFTLGGSPYP GVPVEELFKL LKEGHRMDKP 721 SNCTNELYMM MRDCWHAVPS QRPTFKQLVE DLDRIVAMTS NQEYLDLSVP LDQYSPGFPA 781 TRSSTCSSGE DSVFSHDPLP DEPCLPRCPP HSHGALKRH

ZPAからはShh Shh（sonic hedgehog）、chick 10 20 30 40 50 60 1 MVEMLLLTRI LLVGFICALL VSSGLTCGPG RGIGKRRHPK KLTPLAYKQF IPNVAEKTLG 61 ASGRYEGKIT RNSERFKELT PNYNPDIIFK DEENTGADRL MTQRCKDKLN ALAISVMNQW 121 PGVKLRVTEG WDEDGHHSEE SLHYEGRAVD ITTSDRDRSK YGMLARLAVE AGFDWVYYES 181 KAHIHCSVKA ENSVAAKSGG CFPGSATVHL EHGGTKLVKD LSPGDRVLAA DADGRLLYSD 241 FLTFLDRMDS SRKLFYVIET RQPRARLLLT AAHLLFVAPQ HNQSEATGST SGQALFASNV 301 KPGQRVYVLG EGGQQLLPAS VHSVSLREEA SGAYAPLTAQ GTILINRVLA SCYAVIEEHS 361 WAHWAFAPFR LAQGLLAALC PDGAIPTAAT TTTGIHWYSR LLYRIGSWVL DGDALHPLGM 421 VAPAS ピンクはシグナルペプチド。C末端側のドメイン(Shh-C) がプロテアーゼ活性を持ち，自己消化により生じる約 20 k. DaのN末端側の断片(Shh-N、水色の部分)がShh の活性を担っている。

Shh-Nは受容体と結合 Shh-Nの受容体はPatchedという（chick） 　 10 20 30 40 50 　　　　60 70 80 90 100 1 MASAADALEP ESGSSTAGGG SHPVRAARSA RGRRRRSGGT RRAAAPDREY LQRPSYCDAA FALEQIAKGR ATGRRAPLWL RAKFQRLLFN LGCYIQKNCG 101 KFLVVGLLYS AFAVGLRAAN LETNVEELWV EVGGRVSREL NYTRQKIGEE AMFNPQLMIQ TPQEDGTNVL TTEALRQHLD SALQASRVHV YMYNRQWKLE 201 HLCYKSGELI TEAGYMDQII EYLYPCLIIT PLDCFWEGAK LQSGTAYLLG KPPLQWINFD PLEFLEELKK INYQVESWEE MLNKAEVGHG YMDRPCLNPA 301 DPDCPITAPN KNSTKPLDVA LVLSGGCYGL SRKYMHWQEE LIIGGTVKNS SGKLVSAQAL QTMFQLMTPK QMYEHFKGYE YVSHINWNED KAAAILEAWQ 401 RMYVEVVHQS VAQNSTQKVL SFTTTTLDDI LKSFSDVSVI RVASGYLLML AYACLTMLRW DCAKSQGAVG LAGVLLVALS VAAGLGLCSL IGISFNAATT 501 QVLPFLALGV GVDDVFLLAH AFSETGQNKR IPFEDRTGEC LKRTGASVAL TSISNVTAFF MAALIPIPAL RAFSLQAAVV VVFNFAMVLL IFPAILSMDL 601 YRREDRRLDI FCCFTSPCVT RVIQIEPQAY AENDNICYSS PPPYSSHSFA HETQITMQST VQLRTEYDPH TQAYYTTAEP RSEISVQPVT VTQDSLSCQS 701 PESASSTRDL LSQFSDSSVH CLEPPCTKWT LSTFAEKHYA PFLLKPKAKV VVIFLFLGLL GLSLYGTTRV RDGLDLTDIV PRDTREYDFI AAQFKYFSFY 801 NMYIVTQKAD YPNVQHLLYE LHRSFSNVTY VLLEGDRQLP KMWLHYFRDW LQGLQDAFDS DWETGKITYS NYKNGSDDAV LAYKLLVQTG NRAKPIDISQ 901 LTKQRLVDAD GIINPNAFYI YLTAWVSNDP VAYAASQANI RPHRPEWVHD KADYMPETRL RIPAAEPIEY AQFPFYLNGL RETSDFVEAI EKVRAICNNY 1001 TSLGIASYPN GYPFLFWEQY IGLRHWLLLS ISVVLACTFL VCALFLLNPW TAGIIVVVLA LMTVELFGMM GLIGIKLSAV PVVILIASVG IGVEFTVHIA 1101 LAFLTAIGDK NRRAVLALEH MFAPVLDGAV STLLGVLMLA GSEFDFIVRY FFAVLAILTI LGVLNGLVLL PVLLSFFGPY PEVSPACGRN RLPTPSPEPP 1201 PSIVRFALPP GHTNNGSDSS DSEYSSQTTV SGISEELHHY EATQSPGIPV HQVVVEATEN PVFARSTVVQ PESRHQSSPR LQSNPEAGTQ QVWHQGRQPK 1301 QEVREGLRPP PYRPRRDAFE ISTEGHSGPS NKDRLNHKAH SHNMRSPAFG AMGVPGSAYC QPITTVTASA SVTVAVHPAV HSHNSCRGSF PSCEEYNEDD 1401 RGMFEDPHVP FNVRCERRNS KVEVIELQDV ECEERTAGKI SE 膜貫通部を 12個もつ

受容体に結合すると、、 SmoothendというGPCRを活性化する 10 20 30 40 50 60 1 GPCWLWALAL GLALGPRRCP AAPLNASAAP PERCRRPAAC ERLRFGSCLG SALPYAHTST 61 LLAADSGSQE EAHGKLLLWS GLRNAPRCWD VIQPLLCAVY MPKCEDGQVE LPSQTLCQAT 121 RAPCTIVERE RGWPDFLKCT PDRFPEGCPN EVQNIKFNSS GQCEAPLVRT YNPKSWYEDV 181 EGCGIQCQNP LFTETEHREM HVYIAFSSVT ISCTFFTLAT FVADWRNSNR YPAVILFYVN 241 ACFFVGSIGC VAQFMDGARD EIVCRADGTM RLGEPTSNET LSCVIIFVIV YYSLMSGVIW 301 FVMLTYAWHT SFKALGTTYQ PLLGKTSYFH LITWSIPFVL TVAILAVAQV DGDSVSGICF 361 VGYKNYRYRA GFVLAPIGLV LIVGGYFLIR GVMTLFSIKS NHPGLLSEKA ASKINETMLR 421 LGIFGFLAFG FVFITFGCHF YDFFNQAEWE RSFREYVLCE ANVTIATQTN KPIPECEIKN 481 RPSLLVEKIN LFAMFGTGIS MSTWVWTKAT LLIWKRTWCR LTGQSDDQPK RIKKSKMIAK 541 AFSKRKELLR DPGRELSFSM HTVSHDGPVA GLAFDINEPS ADVSSAWAQH VTKMVARRGA 601 ILPQDVSVTP VATPVPPEER SNLWVVEADV SPELQKRSRK KKRRKKKKEE VCPERRAGLS 661 VAPLTPSSVP RLPRLPQQPC LVAIPRHRGD TFIPTVLPGL SNGAGGLWDG RRRAHVPHFI 721 TNPFCPESGS PEDEENPGPS VGHRQHNGGR RWPPEPLPGG SGVTRTRGRR AGLAPIHSRT 781 NLVNAELLDA DLDF ７回膜貫通型

See you next term.