HPLC Installation Petro van Poppel Field Support Specialist

  • Slides: 24
Download presentation
HPLC Installation Petro van Poppel – Field Support Specialist March 2010

HPLC Installation Petro van Poppel – Field Support Specialist March 2010

Installation Location Hardware Software Personnel

Installation Location Hardware Software Personnel

Installation Location • Safety requirements • Environment • Bench space • Power • Waste

Installation Location • Safety requirements • Environment • Bench space • Power • Waste • IT • Boxes

Installation Hardware • Check all boxes with customer • Plumbing • Wiring • Configuration

Installation Hardware • Check all boxes with customer • Plumbing • Wiring • Configuration • Gas supply • Calibrations • Test solvents and chemicals

Installation Software • Running Software • Drivers • Firmware • Network • Correct level

Installation Software • Running Software • Drivers • Firmware • Network • Correct level of access

Installation Personnel • You (& co) • Future operator • IT administrator • Sign

Installation Personnel • You (& co) • Future operator • IT administrator • Sign off (Manager)

Installation Pre-Installation Manual Checklist

Installation Pre-Installation Manual Checklist

Wiring

Wiring

Plumbing

Plumbing

Plumbing SOLVENT Low Pressure PUMP A/S High Pressure COLUMN Detector Low Pressure F/C

Plumbing SOLVENT Low Pressure PUMP A/S High Pressure COLUMN Detector Low Pressure F/C

Software

Software

Problems Rules of Thumb: • Change only one thing at a time when isolating

Problems Rules of Thumb: • Change only one thing at a time when isolating a LC problem • A problem isn’t really a problem until it occurs at least twice • Reinstall old parts if the substituted new part does not solve the problem • Always write down what you have done or changed for future reference

Problems Chemistry • Data Quality / Accuracy • Chromatography • Methodology Hardware • Pressure

Problems Chemistry • Data Quality / Accuracy • Chromatography • Methodology Hardware • Pressure and Leaks • Cleanliness • Hardware failures • Communications • Location • Short shipment

Chemistry Problems • Need to question the accuracy and precision of method and /

Chemistry Problems • Need to question the accuracy and precision of method and / or analyst. • The chromatography might not work due to bad quality solvents, bad (pre-)column, wrong solvents. • There may be a problem with sample preparation.

Hardware Problems • Low pressure indicates leak in system, high pressure indicates obstruction in

Hardware Problems • Low pressure indicates leak in system, high pressure indicates obstruction in flow path • DOA hardware = replace, but do check simple things • Calibration may be required • LC looks at low quantities.

Pressure Problems • No pressure or flow • Pressure but no flow • Erratic

Pressure Problems • No pressure or flow • Pressure but no flow • Erratic pressure • Leaks

Cleanliness • Use Hipersolv Chemicals only • Use LC grade water, filtered over 0.

Cleanliness • Use Hipersolv Chemicals only • Use LC grade water, filtered over 0. 20µM filter • Avoid contamination by other chemicals or bacterial growth • Replace (pre-) column in time • Clean or replace flow cell in time • Only use LC grade materials

Hardware Failures Pump piston • Seals (in compatible, worn) • Breakage (visual check) •

Hardware Failures Pump piston • Seals (in compatible, worn) • Breakage (visual check) • Piston scoring (replacement seal no good) • Piston seizing (pump overheats, no flow) • (springs broken)

Hardware Failures Flow cell • Bubbles • In-compatible solvents • Leak or blockage •

Hardware Failures Flow cell • Bubbles • In-compatible solvents • Leak or blockage • Bad fittings • Contamination causing baseline issues

Hardware Failures Wavelength • Bad wavelength • Calibration problems • Low UV-range • Time

Hardware Failures Wavelength • Bad wavelength • Calibration problems • Low UV-range • Time constant too high • Temperature drift

Communications Problems • Incorrect interface in software • Bad cables • Instrument not (correct)

Communications Problems • Incorrect interface in software • Bad cables • Instrument not (correct) configured • Lack of access on PC or network • Bad PC • Wrong driver, firmware or combination • Faulty instrument

Location Problems • Unsuitable bench • Power not filtered or protected • Room not

Location Problems • Unsuitable bench • Power not filtered or protected • Room not temperature controlled • Corrosive environment • Other incompatible instruments close by

Summary • Pre-installation checklist • Follow the instructions • Homework • When problems, look

Summary • Pre-installation checklist • Follow the instructions • Homework • When problems, look for simple things : • Leaks • Hardware • Location • Cleanliness