HPLC Installation Petro van Poppel Field Support Specialist
- Slides: 24
HPLC Installation Petro van Poppel – Field Support Specialist March 2010
Installation Location Hardware Software Personnel
Installation Location • Safety requirements • Environment • Bench space • Power • Waste • IT • Boxes
Installation Hardware • Check all boxes with customer • Plumbing • Wiring • Configuration • Gas supply • Calibrations • Test solvents and chemicals
Installation Software • Running Software • Drivers • Firmware • Network • Correct level of access
Installation Personnel • You (& co) • Future operator • IT administrator • Sign off (Manager)
Installation Pre-Installation Manual Checklist
Wiring
Plumbing
Plumbing SOLVENT Low Pressure PUMP A/S High Pressure COLUMN Detector Low Pressure F/C
Software
Problems Rules of Thumb: • Change only one thing at a time when isolating a LC problem • A problem isn’t really a problem until it occurs at least twice • Reinstall old parts if the substituted new part does not solve the problem • Always write down what you have done or changed for future reference
Problems Chemistry • Data Quality / Accuracy • Chromatography • Methodology Hardware • Pressure and Leaks • Cleanliness • Hardware failures • Communications • Location • Short shipment
Chemistry Problems • Need to question the accuracy and precision of method and / or analyst. • The chromatography might not work due to bad quality solvents, bad (pre-)column, wrong solvents. • There may be a problem with sample preparation.
Hardware Problems • Low pressure indicates leak in system, high pressure indicates obstruction in flow path • DOA hardware = replace, but do check simple things • Calibration may be required • LC looks at low quantities.
Pressure Problems • No pressure or flow • Pressure but no flow • Erratic pressure • Leaks
Cleanliness • Use Hipersolv Chemicals only • Use LC grade water, filtered over 0. 20µM filter • Avoid contamination by other chemicals or bacterial growth • Replace (pre-) column in time • Clean or replace flow cell in time • Only use LC grade materials
Hardware Failures Pump piston • Seals (in compatible, worn) • Breakage (visual check) • Piston scoring (replacement seal no good) • Piston seizing (pump overheats, no flow) • (springs broken)
Hardware Failures Flow cell • Bubbles • In-compatible solvents • Leak or blockage • Bad fittings • Contamination causing baseline issues
Hardware Failures Wavelength • Bad wavelength • Calibration problems • Low UV-range • Time constant too high • Temperature drift
Communications Problems • Incorrect interface in software • Bad cables • Instrument not (correct) configured • Lack of access on PC or network • Bad PC • Wrong driver, firmware or combination • Faulty instrument
Location Problems • Unsuitable bench • Power not filtered or protected • Room not temperature controlled • Corrosive environment • Other incompatible instruments close by
Summary • Pre-installation checklist • Follow the instructions • Homework • When problems, look for simple things : • Leaks • Hardware • Location • Cleanliness
- Marketingplan inhoud
- Nhs learning support fund specialist subject payment
- Federal field specialist orr
- Petro-tech heat technology inc
- Opw fuel management systems
- Maria petro teacher
- Petro kononova
- Petrooxy
- Petro fast
- Petro pariñas sac houston
- Sutura petro giugulare
- Bruce a research chemist for a major petrochemical company
- Minor and major details
- Magnitude of magnetic force
- Individual differences factors
- Field dependent vs field independent
- Electric field and magnetic field difference
- E field h field
- Data types and field properties
- Field dependent definition
- Magnetic field
- Band broadening in hplc
- Priming in hplc
- Rührapparatur aufbau
- Hplc werking