Horizon Chromatography HPLCUHPLC Columns Introduction Horizon new HPLC

Horizon Chromatography HPLC/UHPLC Columns

Introduction • Horizon new HPLC, UHPLC and Core-Shell ranges • • Ultra pure silica 99. 999% High surface area (300 m 2/g) Excellent peak shapes High resolution selectivity choices • We pride ourselves on our delivery and sales service • Technical Support service

Available Phases Aurashell – Core-Shell Horizon – Fully Porous (300 m 2/g) • • • Aurashell C 18/PFP Aurashell Aqua C 18 Aurashell HILIC • Aurashell PEP C 18 (160Å) • Aurashell PRO C 4 (300Å) Horizon C 18/PFP Horizon Aqua C 18 Horizon C 8 Horizon HILIC Horizon Phenyl Horizon Amino Horizon Cyano

Physicals • Ultra pure 99. 999% silica template • All phases are extensively endcapped • Horizon, porous particles – 1. 6µm UHPLC particles – 3µm High efficiency particle – 5µm standard ‘work horse’ particle • Aurashell, core-shell – 2. 7µm ultra high efficiency, low backpressure – 5µm high efficiency, low backpressure

Physicals S. A. %C Pore Size p. H range USP Purity Endcapped Horizon C 18 300 m 2/g 20 120Å 1 -11 L 1 99. 999% Yes Horizon Aqua 300 m 2/g 16 120Å 2 -10 L 1 99. 999% Yes Horizon C 18/PFP 300 m 2/g 15 120Å 2 -10 L 1 99. 999% Yes Horizon C 8 300 m 2/g 12 120Å 2 -10 L 7 99. 999% Yes Horizon Phenyl 300 m 2/g 12 120Å 2 -8 L 11 99. 999% Yes Horizon Cyano 300 m 2/g 7 120Å 1 -6 L 10 99. 999% Yes Horizon HILIC 300 m 2/g 4 120Å 2 -8 L 20 99. 999% Yes Horizon Amino 300 m 2/g 5 120Å 2 -8 L 8 99. 999% Yes

Aurashell • Aurashell – Monodisperse core shell particle – High speed separations – Uniform packed bed, low backpressure – Multiple particle sizes • 2. 7µm • 5µm • Reversed phase chemistries • Bioanalysis optimised phases

UHPLC • 1. 6µm Horizon particles for UHPLC – Fully porous ultra pure inert silica – Excellent peak shapes and reproducibility – High efficiency UHPLC results – Multiple orthogonal selectivity's available

Horizon C 18 • Horizon C 18 – HPLC and UHPLC particle sizes – Excellent performance general purpose C 18 alkyl chain stationary phase with high stability – Excellent peak shapes – Excellent selectivity – Guaranteed batch to batch reproducibility

Horizon C 18 • Horizon C 18 – Ultra high purity silica 99. 999% – 300 m 2/g surface area – 120Å pore size – p. H range 1 -11 – USP : L 1

Horizon C 18 Column: Horizon C 18 3µm 150 x 4. 6 mm Mobile Phase: A: 10 m. M Ammonium Bicarbonate p. H 10 B: Me. OH Gradient : 60: 40 – 100% B in 10 minutes Flow rate : 1 ml/min Wavelength: 220 nm Temperature: 30 o. C 1. Tramadol 2. Amitriptyline

Horizon C 18 Column: Horizon C 18 3µm 150 x 4. 6 mm Mobile Phase: A: 10 m. M Ammonium formate p. H 3. 5 B: Me. CN Gradient : 60: 40 – 100% B in 10 minutes Flow rate : 1 ml/min Wavelength: 254 nm Temperature: 20 o. C 1. Rosuvastatin 2. Simvastatin 3. Atorvastatin

Horizon C 18/PFP • Horizon C 18/PFP – HPLC and UHPLC particle sizes – – Alternative selectivity L 1 columns Offers strong hydrophobic, pi-pi and dipole interactions Extend retention of halogenated compounds Guaranteed batch to batch reproducibility

Horizon C 18/PFP • Horizon C 18/PFP – Ultra high purity silica 99. 999% – 300 m 2/g surface area – 120Å pore size – p. H range 2 -10 – USP : L 1

Horizon C 18/PFP Column: Mobile Phase: Flow rate : Wavelength: Temperature: 1, 2, 3 4 3µm 150 x 4. 6 mm 50: 50 Water: Me. OH 1 ml/min 254 nm 40 o. C 5, 6 Traditional C 18 7 8 3 4 5 Horizon C 18/PFP 6 2 1 7 8 1. 2. 3. 4. 5. 6. 7. 8. 1, 2, 3 -Trimethoxybenzene 1, 2, 4 -Trimethoxybenzene 1, 2 -Dimethoxybenzene 1, 4 -Dimethoxybenzene Methoxybenzene 1, 3 -Dimethoxybenzene 1, 3, 5 -Trimethoxybenzene Toluene

Horizon C 18/PFP Column: Horizon C 18/PFP 3µm 150 x 4. 6 mm Mobile Phase: A: 0. 1% Formic acid B: 0. 1% Formic acid in Me. CN Gradient : 10 – 100% B in 10 minutes Flow rate : 1 ml/min Wavelength: 254 nm Temperature: 20 o. C 1. 2. 3. 4. Paracetamol Hydrochlorothiazide Methylphenylsulfoxide Methylphenylsulfone

Horizon AQUA C 18 • Horizon Aqua C 18 – HPLC and UHPLC particle sizes – Excellent performance C 18 with capability to operate in 100% aqueous mobile phases – Resistant to retention loss caused by highly aqueous mobile phases – Analyse polar compounds

Horizon AQUA C 18 • Horizon Aqua C 18 – Ultra high purity silica 99. 999% – 300 m 2/g surface area – 120Å pore size – p. H range 2 -10 – USP : L 1

Horizon AQUA C 18 Column: Horizon Aqua C 18 5µm 150 x 4. 6 mm Mobile Phase: A: 50 m. M KH 2 PO 4 p. H 3. 5 B: Me. OH Gradient : 0 – 100% B in 15 minutes Flow rate : 1 ml/min Wavelength: 205 nm Temperature: 30 o. C 1. 2. 3. 4. 5. 6. 7. 8. Vitamin B 1 Vitamin B 6 Vitamin B 3 Vitamin B 5 Folic acid Vitamin B 12 Vitamin H Vitamin B 2

Horizon AQUA C 18 Column: Horizon Aqua C 18 5µm 150 x 4. 6 mm Mobile Phase: A: 0. 1% TFA B: 0. 1% TFA in Me. OH Gradient : 0 – 100% B in 15 minutes Flow rate : 1 ml/min Wavelength: 270 nm Temperature: 20 o. C 1. Dopamine 2. Serotonin 3. DOPAC

Peptide/Protein Columns • Horizon BIO phases – Horizon fully porous PEP C 18 and PRO C 4 • PEP C 18 = 200Å C 18 designed for peptide analysis and mapping • PRO C 4 = 300Å C 4 for wide range of protein analysis • Aurashell PEP C 18 – 2. 7µm core shell – 160Å pore size – High efficiency phase for peptides • Aurashell PRO C 4 – 3. 5µ, core shell – 300Å pore size – High efficiency phase for proteins

Aurashell PEP C 18 • Aurashell PEP C 18 – Monodisperse – 160Å pore size – C 18 ligand (6% C) – p. H range 1 -9 – USP : L 1

Aurashell PEP C 18

Aurashell PEP C 18 Tryptic digest 2. 7µm Aurashell PEP C 18 150 x 2. 1 mm 60 minute gradient 30 minute gradient 20 minute gradient

Aurashell PRO C 4 • Aurashell PRO C 4 – Monodisperse – 300Å pore size – C 4 ligand (1% C) – p. H range 1 -9 – USP : L 26

Aurashell PRO C 4

Aurashell PRO C 4 Protein Mix 3. 5µm Aurashell PRO C 4 100 x 2. 1 mm Ribonuclease A (13. 7 k. Da) Cytochrome C (12 k. Da) Halo-Transferrin (77 k. Da) Apomyoglobin (17 k. Da)

Other Dimensions • Preparative Columns • 10 mm or 21. 2 mm diameters – Ultra High purity 5µm or 10µm particles – High surface area • High sample loading • Excellent sample recovery • Capillary 75µm i. d. – Available with Horizon or Aurashell particles – Full range of bonded phases – Standard and custom made lengths available

Guards and Filters Horizon Guards: - Significantly increase the lifetime of columns - Prevent contamination - Available in all phase chemistries Horizon Analytical pre-column filter: - 2µm porosity frit - 400 bar pressure rated - Finger tight Horizon UHPLC pre-column filter: - 0. 5µm porosity frit - 1000 bar pressure rated - Compatible with all UHPLC systems

Conclusion • Horizon phases offer: – Selectivity – Efficiency – Excellent peak shapes – UHPLC 1. 6µm particles • Aurashell phases offer: – High Efficiency – High Resolution – High Speed

Contact Us E-mail : info@horizonhplc. com www. horizonhplc. com