Highly sensitive and specific in situ hybridization assay

Highly sensitive and specific in situ hybridization assay for quantification of SOX 11 m. RNA in mantle cell lymphoma reveals association of TP 53 mutations with negative and low SOX 11 expression by Birgit Federmann, Leonie Frauenfeld, Helga Pertsch, Vanessa Borgmann, Julia Steinhilber, Irina Bonzheim, Falko Fend, and Leticia Quintanilla-Martinez haematol Volume 105(3): 754 -764 March 1, 2020 © 2020 by Ferrata Storti Foundation

Clinical and histological data of the 66 cases. Birgit Federmann et al. Haematologica 2020; 105: 754 -764 © 2020 by Ferrata Storti Foundation

Illustration of the staining pattern in the different RNAscope scores and correlation to the realtime quantitative PCR (RT-q. PCR) results, which shows a decrease of SOX 11 -m. RNA levels with decreasing staining scores. Birgit Federmann et al. Haematologica 2020; 105: 754 -764 © 2020 by Ferrata Storti Foundation

SOX 11 RNAscope and RT-q. PCR analyses in the three MCL subgroups. Birgit Federmann et al. Haematologica 2020; 105: 754 -764 © 2020 by Ferrata Storti Foundation

Mantle cell lymphoma (MCL) with splenic involvement and plasmacytoid differentiation (case #9). Birgit Federmann et al. Haematologica 2020; 105: 754 -764 © 2020 by Ferrata Storti Foundation

Classical cyclin D 1 negative mantle cell lymphoma (D 1 -MCL) with mantle zone pattern (case #10). Birgit Federmann et al. Haematologica 2020; 105: 754 -764 © 2020 by Ferrata Storti Foundation

Histological, immunohistochemical and molecular data of 13 cases with TP 53 mutation. Birgit Federmann et al. Haematologica 2020; 105: 754 -764 © 2020 by Ferrata Storti Foundation

Mantle cell lymphoma (MCL) with blastoid morphology, low SOX 11 expression and TP 53 mutation (case # 58). Birgit Federmann et al. Haematologica 2020; 105: 754 -764 © 2020 by Ferrata Storti Foundation

Leukemic non-nodal disease (nn. MCL) mantle cell lymphoma (MCL) (case #15). Birgit Federmann et al. Haematologica 2020; 105: 754 -764 © 2020 by Ferrata Storti Foundation