Hematopoietic stem cells in coculture with mesenchymal stromal

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Hematopoietic stem cells in co-culture with mesenchymal stromal cells - modeling the niche compartments

Hematopoietic stem cells in co-culture with mesenchymal stromal cells - modeling the niche compartments in vitro by Duohui Jing, Ana-Violeta Fonseca, Nael Alakel, Fernando A. Fierro, Katrin Muller, Martin Bornhauser, Gerhard Ehninger, Denis Corbeil, and Rainer Ordemann haematol Volume 95(4): 542 -550 April 8, 2010 © 2010 by Ferrata Storti Foundation

Distinct compartmentalization of HSC cultured on MSC. (A, B) Scanning electron microscopy analysis revealed

Distinct compartmentalization of HSC cultured on MSC. (A, B) Scanning electron microscopy analysis revealed that HSC are either above the MSC layer (black asterisks) or beneath (white arrow). Duohui Jing et al. Haematologica 2010; 95: 542 -550 © 2010 by Ferrata Storti Foundation

Cell proliferation and cell cycle status. Duohui Jing et al. Haematologica 2010; 95: 542

Cell proliferation and cell cycle status. Duohui Jing et al. Haematologica 2010; 95: 542 -550 © 2010 by Ferrata Storti Foundation

Immunophenotype of the cells in the three compartments. Duohui Jing et al. Haematologica 2010;

Immunophenotype of the cells in the three compartments. Duohui Jing et al. Haematologica 2010; 95: 542 -550 © 2010 by Ferrata Storti Foundation

Tracking cell division in the three distinct compartments using CFSE staining. Duohui Jing et

Tracking cell division in the three distinct compartments using CFSE staining. Duohui Jing et al. Haematologica 2010; 95: 542 -550 © 2010 by Ferrata Storti Foundation

(A) Enrichment of CD 34+CD 38−cells in the phase-dim fraction. Duohui Jing et al.

(A) Enrichment of CD 34+CD 38−cells in the phase-dim fraction. Duohui Jing et al. Haematologica 2010; 95: 542 -550 © 2010 by Ferrata Storti Foundation

Graphic of the in vitro HSC/MSC co-culture system. Duohui Jing et al. Haematologica 2010;

Graphic of the in vitro HSC/MSC co-culture system. Duohui Jing et al. Haematologica 2010; 95: 542 -550 © 2010 by Ferrata Storti Foundation

Hematopoietic stem cells in coculture with mesenchymal stromalHematopoietic stem cells in coculture with mesenchymal stromal
Gastrointestinal Stromal TumoursGIST INTRODUCTION Stromal or mesenchymal tumorsGastrointestinal Stromal TumoursGIST INTRODUCTION Stromal or mesenchymal tumors
Mesenchymal stromal cells transiently alter the inflammatory milieuMesenchymal stromal cells transiently alter the inflammatory milieu
Pancreasderived mesenchymal stromal cells share immune responsemodulating andPancreasderived mesenchymal stromal cells share immune responsemodulating and
Cardiac Nestin Mesenchymal Stromal Cells Enhance Healing ofCardiac Nestin Mesenchymal Stromal Cells Enhance Healing of
Bone marrow mesenchymal stromal cells induce nitric oxideBone marrow mesenchymal stromal cells induce nitric oxide