Hematopoiesis Simplified Part 1 Erythropoiesis Larry Johnson Texas

Hematopoiesis Simplified: Part 1 Erythropoiesis Larry Johnson Texas A&M University

Hematopoiesis Simplified: Part 1 Erythropoiesis Objectives are to: • Identify the developmental cells of erythropoiesis • Characterize developmental changes in the cytoplasm and nucleus of these cells Bone marrow 32583 Blood vessel

19761 Bone marrow Blood in vessel

Peripheral blood Erythrocytes Reticulocytes 21

Erythropoiesis Erythrocytes No nucleus and red cytoplasm Reticulocytes No nucleus and blue - grey cytoplasm Orthochromatophilic erythroblasts Polychromatophilic erythroblasts Dark, small, spherical nucleus blue – grey cytoplasm Darkening, fractured , spherical nucleus and mixed pools of grey and blue cytoplasm Basophilic erythroblasts Fractured, spherical nucleus and thin rim of dark blue cytoplasm Proerythroblasts Uniformly light, spherical nucleus with thin rim of medium to dark blue cytoplasm

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Erythropoiesis Erythrocytes Proerythroblasts Reticulocytes Orthochromatophilic erythroblasts Basophilic erythroblasts Polychromatophilic erythroblasts Erythropoiesis

Orthochromatophilic erythroblasts Erythrocytes

Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis

Polychromatophilic erythroblasts

Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis

Basophilic erythroblast

Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis

Proerythroblasts

Erythropoiesis Erythrocytes Reticulocytes Orthochromatophilic erythroblasts

Early orthochromatophilic erythroblasts Late polychromatophilic erythroblasts

Polychromatophilic erythroblasts Early polychromatophilic erythroblasts

Basophilic erythroblasts Proerythroblasts

ut 112 Orthochromatophilic erythroblasts RBCs Late polychromatophilic erythroblast Basophilic erythroblast platelets Polychromatophilic erythroblast Reticulocyte Erythrocytes Eosinophil or basophil Proerythroblast

Bone marrow Reticulocyte entering blood vessel Blood vessel 32583

The End of Hematopoiesis Simplified: Part 1 Erythropoiesis

• Granulopoiesis is next

Hematopoiesis Simplified: Part 2 Granulopoiesis Larry Johnson Texas A&M University

Hematopoiesis Simplified: Part 2 Granulopoiesis Objectives are to: • Identify the developmental cells of granulopoiesis • Characterize developmental changes in the cytoplasm and nucleus of these cells 32583 Bone marrow

19761 Bone marrow Blood

32583 ` Bone marrow Developing granulocytes

Peripheral blood 21

Granulopoiesis Neutrophils Lobulated, dark nucleus, mature grey cytoplasm with small granules Neutrophilic band or stab cells Horse-shoe shaped, darkening nucleus, grey mature cytoplasm Neutrophilic metamyelocytes Oval to kidney bean-shaped, indented nucleus, grey mature cytoplasm with a weak blue tent Neutrophilic myelocytes Large, oval, non-indented nucleus, large amount of cytoplasm with specific and non-specific granules Promyelocytes Large, oval, non-indented nucleus, large amount of sky blue cytoplasm with non-specific granules

Granulopoiesis Neutrophils Promyelocytes Neutrophilic band cells Neutrophilic myelocytes Neutrophilic metamyelocytes

ut 112 Neutrophilic metamyelocytes Neutrophilic metamyelocyte Platelets Eosinophil or basophil Neutrophilic myelocytes

Neutrophils Promyelocytes Neutrophilic band cells Neutrophilic myelocytes Neutrophilic metamyelocytes Granulopoiesis

Neutrophilic metamyelocytes Lymphocytes Neutrophilic myelocytes Unknown nuclei Promyelocyte Lymphocytes Neutrophilic myelocytes Neutrophilic band cells Neutrophilic metamyelocytes Neutrophilic band cells

Neutrophilic band cells Neutrophils Granulopoiesis

Neutrophilic metamyelocytes Early neutrophilic metamyelocytes

Neutrophilic myelocytes

Late promyelocytes Promyelocyte

Neutrophilic metamyelocytes Unknown nuclei Neutrophilic myelocytes Neutrophilic band cells Proerythroblast Orthochromatophilic erythroblasts Eosinophils or basophils Neutrophilic metamyelocytes Orthochromatophilic erythroblasts

Bone marrow 32583 White blood cell entering a blood vessel Blood vessel

The End of Hematopoiesis Simplified: Part 2 Granulopoiesis