Group Name MRSA 2 Group member name Group

Group Name MRSA 2 Group member name Group member name Workshop on Whole Genome Sequencing and Analysis, 27 -29 Mar. 2016

Isolates Sample name ERR 070040 -P 12 ERR 124435 -P 24 ERR 124434 -P 22 ERR 128716 -HW ERR 070035 -notoutbreak Subtype Notes ST 2371 From Patient 12 ST 2371 From Patient 24 ST 2371 From Patient 22 From Health Care Worker - misses the erm. C ST 2371 plasmid ST 8 Not part of the outbreak

Result of species identification (Kmer. Finder) Strain ERR 070040 -P 12 ERR 124435 -P 24 ERR 124434 -P 22 ERR 128716 -HW ERR 070035 -notoutbreak Species acc. Predicted to Harris et al species 2013 Closest matching strain Template coverage

Representative Kmer. Finder result

Result of Multilocus Sequence Typing (MLST) Strain ERR 070040 -P 12 ERR 124435 -P 24 ERR 124434 -P 22 ERR 128716 -HW ERR 070035 -notoutbreak ST acc. to Harris Predicted ST et al 2014

Representative Multilocus Sequence Typing result

Result of antibiotics resistance analysis Cefoxitin/M Ciproethicillin floxacin (Mec. A) (chromosomal mutation, Isolate nor. A) Erythromycin (Erm(A), Erm(B), Erm(C)) Fusidic acid Gentamicin Mupirocin (chromo(aac(6’)(chromosomal aph(2'')) somal mutation) Rifampicin (chromosomal mutation) Tetra-cycline Vancomycin (tet. C, tet. M, (Chromotet. R, tet. S somal mutations or the van. A or van. B gene cluster. ) P 12 P 24 P 22 HW non-outbreak How is the agreement with the results presented in Harris et al 2013? Neomycin (aad. D, aac(6')aph(2''))

Result of plasmid analysis Isolate rep 10 rep. US 12 rep 20 rep 5 ERR 070040 P 12 ERR 124435 P 24 ERR 124434 P 22 ERR 128716 HW ERR 070035 notoutbreak For each strain write which node the replicon was found on. If not found, leave the cell empty. Which plasmid is the erm. C gene on? rep 7

Distance matrix made by NDtree and tree ma

Distance matrix made by CSIPhylogeny and tr