GratingCoupled SPR for rapid labelfree arraybased sensing 20040630
Grating-Coupled SPR for rapid, label-free, arraybased sensing 指導教授:林啟萬 研究生:蘇明啟 日期: 2004/06/30
outline n n n Basis of grating-coupled SPR Structure of GC-SPR and Schottky Junction An experiment of GC-SPR Array-based GC-SPR Conclusion Reference
How does grating-coupled SPR occur The sensitivity of prism-coupled SPR sensor is a little higher than that of grating-based SPR sensor. However, GCSPR has advantages of small size, cheap, suitable for portable design.
The fabrication process of grating I. II. 1. Start with fabrication by holographically with an blue laser (488 nm) on Ga. As substrate with doping level from 5 x 1016 to 5 x 1018 cm-3 1. Exposing Shipley S 1405 -17 photoresist to two expanded collimated interfering beams of 457. 9 nm radiation from an argon ion laser. 2. The microstructure was revealed by AFM, which reconstructs the grating profile and determines groove depth and grating period. 2. Hardened by exposure to ultraviolet radiation 3. Thermally evaporated metal (Au) films were deposited at room temperature. 3. Etched by an energetic atom beam to transfer the spatial profile in the photoresist through into the silica substrates.
Band Diagram of a Metal. Semiconductor Junction Each material has a characteristic energy which is needed to remove an electron from its surface to infinity. This is called the work function of the material. When a high work function metal comes in contact with a semiconductor with a lower work function, electrons flow from the semiconductor to the metal. This results in a band configuration shown in Figure.
Schematic Diagram of GCSPR period d=652 nm height h~18 nm Incident angle of P-polarized light is 18˚
alcohol concentration vs angular shift Alcohol concentration in water (%) 1. 0 2. 7. 3 3. 13. 6 4. 19. 2 5. 24. 0 6. 28. 3 incident angle vs short-circuit photocurrent
Make Use of Gratings on CDROM Plastic molded transducers (grating chips)
Grating-Coupled SPR Period is 867 nm Groove depth is 40 nm
Simultaneous Measurement on Microarrays avidin Biotin MUA+PL Au Film (80 nm) Plastic Grating MUA: 11 -mercaptoundecanoic acid PL: electrostatically adsorbed Poly-L-lysine
SPR Curve: Incident angle vs Reflectance Principle of operating of kinetic analysis system. Light source: 867 nm LED SPR curves collected by CCD camera
Kinetic Calculations
Detection of Protein Binding Spot size 250 um 1 -cm 2 containing 400 spots
Reference n n n n Jennifer M. Brockman, Salvador M. Fernandez, Grating-coupled surface plasmon resonance for rapid, label-free, array-based sensing, American Laboratory, June 2001 N. L. Dmitruk, O. I. Mayeva, S. V. Mamykin, O. B. Yastrubchak, On a Control of Photo-surface Plasmon Coupling at a Multilayer Diffraction Grating, Institute for Physics of Semiconductors NAS of Ukraine, IEEE 2000 Dmitruk N. L. , Mayeva O. I. , Mamykin S. V. , Fursenko O. V. , Yastrubchak O. B. , Influence of Multilayer Coating Peculiarities on Efficiency of Surface Plasmon Resonance Photodetector, Institute of Physics of Semiconductors NAS of Ukraine, IEEE 1998 R A Watts, J R Sambles, M C Hutley, T W Preist, C R Lawrence, A New Optical Technique for Characterizing Reference artefacts for Surface Profilometry, Thin Film Photonics Group, Department of Physics, University of Exeter, UK, National Physical Laboratory, Middlesex UK, Nanotechnology 8 (1997) 35 -39 http: //www. formosams. com http: //nina. ecse. rpi. edu/shur/Ch 3/sld 043. htm http: //solarwww. mtk. nao. ac. jp/kobayash/thesis/node 34. html
Thanks for your attention!
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