Glycolysis Cycle Presented By Miss Sunayana S ahire

Glycolysis Cycle Presented By: Miss. Sunayana S. ahire Asst. Professor, Microbiology Department, K. C. E. P. G. College, Jalgaon.

Introduction � Defination of Glycolysis : Glycolysis is defined as the sequence of reaction converting glucose to pyruvate or lactose with the production of ATP

Silent features of glycolysis � Enzyme location � Process is aeorobical and anaerobic condition � Glycolysis is major pathway for ATP synthesis � Essential for brain

Reaction of glycolysis Ø The 1. 2. 3. pathway can be divided into three distinct phase Energy investment phase Splitting phase Energy generation phase

Energy investment phase � Energy 1. 2. 3. investment phase carry three reactions Phosphorylation Isomerization phosphorylation

Phosphorylation � Glucose is phosphorylated to glucose 6 phosphat by hexokinase � Reaction is irreversible � ATP dependent

Isomerization � Glucose 6 phosphate fructose 6 phosphate � Enzyme phosphohexoisomerase

Phosphorylation � Fructose 6 phosphate 1, 6 bisphosphate � Enzyme phosphofructokinase

Splitting phase � This six carbon sugar is cleaved produce two 3 carbon molecule glyceraldehyde-3 phosphate (GAP) and dihydroaceton phosphate (DHAP) � This reacation is catalyzed by aldolase

Isomerization � Dihydroxyacetone phosphate is oxidized to form glyceraldehyde-3 -phosphate. � This reaction catalyzed by triose phosphate isomerase

Energy generation phase � Two molecule of glyceraldehyde-3 phosphate are oxidized � Glyceraldehyde-3 -phosphate dehydrogenase catalyze the conversion of glyceraldehyde-3 phosphate into 1, 3 bisphoglycerate

Substrate level phosphorylation � The enzyme phosphoglycerate kinase act on 1, 3 bisphoglycerate resulting in the synthesis of ATP and formation 3 phosphoglycerate � This reaction is reversible

� 3 -phosphoglycerate is convertede to 2 phosphoglyecerate by phosphoglycerate mutase

� Thr high energy compound phodphoenolpyruvste is generated to form 2 phosphoglecerate by the enzyme enolase

� The enzyme pyruvate kinase catalyze the transfer of high energy phosphate from phosphoenolpyruvate to ADP leading the formation of ATP � This reaction is irriversible

Production ATP in glycolysis � Under anaerobic condition 2 ATP are synthesize � Under aerobic condition 8 or 6 ATP are synthgesize