Global Coagulation and Thrombin Generation Assays Diapharma University

Global Coagulation and Thrombin Generation Assays Diapharma University M. Sobo info@diapharma. com ML-00 -00633_Rev 01

Intrinsic/Contact Pathway HMWK+Prekallikrein Factor XIIa Extrinsic/Tissue Factor Pathway Wound Factor XIa Tissue Factor VIIa: TF Factor X Factor IXa + Factor VIIIa Factor IX Factor Xa + Factor Va Common Pathway Prothrombin ML-00 -00633_Rev 01 Thrombin Fibrinogen Fibrin Factor XII Abnormal Surface

Feed forward by Thrombin Factor XIa Factor IXa Factor XI + Factor VIIIa Factor IX Factor Xa Factor VIII Factor V + Factor Va Prothrombin ML-00 -00633_Rev 01 Thrombin

Down Regulation Factor IXa + Factor VIIIa Factor Xa + Factor Va Activated Protein C (APC) Prothrombin Thrombin + Thrombomodulin Protein C ML-00 -00633_Rev 01

Prothrombin Time (PT) + Thromboplastin + Ca++ Time to Clot FVII FX FV FII ML-00 -00633_Rev 01 Normal: ~12 seconds Factor Deficiency: >15 seconds

Activated Partial Thromboplastin Time (a. PTT) + Surface Activator(s) + Phospholipids Time to Clot FVIII FX FIX FXI FV FXII FII ML-00 -00633_Rev 01 Normal: 30 -50 seconds Factor Deficiency: >50 seconds

+ Trigger 400 + Fluorescent Reporter 350 Peak Thrombin Ina 300 on ati v cti Continuous Fluorescent Measurement dex Vel e 150 as ocit y In 200 Ph n. M Thrombin 250 100 AUC 50 0 0 10 Tlag ML-00 -00633_Rev 01 Tmax 20 30 Time (min) 40 *The Thrombin Generation Assay is for Research Use Only in the US and Canada 50

Global Coagulation and Thrombin Generation Assay Diapharma University M. Sobo info@diapharma. com ML-00 -00633_Rev 01