Genus Staphylococci Grams positive Catalase positive cocci S
![Genus Staphylococci Gram’s positive, Catalase positive cocci Genus Staphylococci Gram’s positive, Catalase positive cocci](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-1.jpg)
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![S. aureus S. epidermidis Skin infections Prosthetic Infection Osteomyelitis Food poisoning Toxic shock syndrome S. aureus S. epidermidis Skin infections Prosthetic Infection Osteomyelitis Food poisoning Toxic shock syndrome](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-3.jpg)
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![STAPHYLOCOCCI • • • Gram positive Facultative anaerobes Grape like-clusters Catalase positive Major components STAPHYLOCOCCI • • • Gram positive Facultative anaerobes Grape like-clusters Catalase positive Major components](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-5.jpg)
![Cultural Characteristics * Aerobic or facultative anaerobic * Grow on (nutrient agar & blood Cultural Characteristics * Aerobic or facultative anaerobic * Grow on (nutrient agar & blood](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-6.jpg)
![Macroscopic characteristics Staphylococcus aureus cause haemolysis on blood agar because of haemolysin enzyme, a Macroscopic characteristics Staphylococcus aureus cause haemolysis on blood agar because of haemolysin enzyme, a](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-7.jpg)
![s. aureus s. epidermidis s. aureus s. epidermidis](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-8.jpg)
![MANNITOL SALT AGAR Na. Cl 7. 5% selective Mannitol differential Staphylococcus aureus can ferment MANNITOL SALT AGAR Na. Cl 7. 5% selective Mannitol differential Staphylococcus aureus can ferment](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-9.jpg)
![Test S. aureus Mannitol fermentation Positive S. epidermidis Negative S. saprophyticus Usually positive Positive Test S. aureus Mannitol fermentation Positive S. epidermidis Negative S. saprophyticus Usually positive Positive](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-10.jpg)
![Specimens 1 Direct Gram’s Slide 2 Biochemical Culture 4 3 Serology Antibiotics sensitivity 5 Specimens 1 Direct Gram’s Slide 2 Biochemical Culture 4 3 Serology Antibiotics sensitivity 5](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-11.jpg)
![Specimens: Pus, Swabs Urine C. S. F. Sputum Blood In Cases Of Septicemia. Specimens: Pus, Swabs Urine C. S. F. Sputum Blood In Cases Of Septicemia.](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-12.jpg)
![Biochemical tests: Coagulase test: DETECT the ability of S. aureus to clot blood plasma Biochemical tests: Coagulase test: DETECT the ability of S. aureus to clot blood plasma](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-13.jpg)
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![Coagulase test Coagulase test](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-15.jpg)
![C o a gu l a s e t e s t -ve +ve C o a gu l a s e t e s t -ve +ve](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-16.jpg)
![Catalase test Positive by bubble formation Catalase test Positive by bubble formation](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-17.jpg)
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![Table summarized the point of differences between 3 imp. Staphylococci species Tests S. aureus Table summarized the point of differences between 3 imp. Staphylococci species Tests S. aureus](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-20.jpg)
![Gelatinase test Nutrient gelatin is a differential medium that tests the ability of an Gelatinase test Nutrient gelatin is a differential medium that tests the ability of an](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-21.jpg)
![Summary: • Genus Staphylococci Gram positive , cocci in shape. • There are 3 Summary: • Genus Staphylococci Gram positive , cocci in shape. • There are 3](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-22.jpg)
![THANKS THANKS](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-23.jpg)
- Slides: 23
![Genus Staphylococci Grams positive Catalase positive cocci Genus Staphylococci Gram’s positive, Catalase positive cocci](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-1.jpg)
Genus Staphylococci Gram’s positive, Catalase positive cocci
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![S aureus S epidermidis Skin infections Prosthetic Infection Osteomyelitis Food poisoning Toxic shock syndrome S. aureus S. epidermidis Skin infections Prosthetic Infection Osteomyelitis Food poisoning Toxic shock syndrome](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-3.jpg)
S. aureus S. epidermidis Skin infections Prosthetic Infection Osteomyelitis Food poisoning Toxic shock syndrome Pneumonia Acute endocarditis Infective arthritis Necrotizing fasciitis Sepsis UTI especially in patient with catheter & low immunity. S. saprophyticus UTI in sexually active females Non Gonococcal Urethritis in males.
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![STAPHYLOCOCCI Gram positive Facultative anaerobes Grape likeclusters Catalase positive Major components STAPHYLOCOCCI • • • Gram positive Facultative anaerobes Grape like-clusters Catalase positive Major components](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-5.jpg)
STAPHYLOCOCCI • • • Gram positive Facultative anaerobes Grape like-clusters Catalase positive Major components of normal flora - skin - nares 5
![Cultural Characteristics Aerobic or facultative anaerobic Grow on nutrient agar blood Cultural Characteristics * Aerobic or facultative anaerobic * Grow on (nutrient agar & blood](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-6.jpg)
Cultural Characteristics * Aerobic or facultative anaerobic * Grow on (nutrient agar & blood agar) & can tolerate 9% Na. Cl. * The colonies are round, smooth, raised, & glistening, producing pigments -Staphylococcus epidermides : gray to white - Staphylococcus aureus : gray to golden – yellow Only Staphylococcus aureus produce β-haemolysis on blood
![Macroscopic characteristics Staphylococcus aureus cause haemolysis on blood agar because of haemolysin enzyme a Macroscopic characteristics Staphylococcus aureus cause haemolysis on blood agar because of haemolysin enzyme, a](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-7.jpg)
Macroscopic characteristics Staphylococcus aureus cause haemolysis on blood agar because of haemolysin enzyme, a zone of β – haemolysis will appear around the colony
![s aureus s epidermidis s. aureus s. epidermidis](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-8.jpg)
s. aureus s. epidermidis
![MANNITOL SALT AGAR Na Cl 7 5 selective Mannitol differential Staphylococcus aureus can ferment MANNITOL SALT AGAR Na. Cl 7. 5% selective Mannitol differential Staphylococcus aureus can ferment](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-9.jpg)
MANNITOL SALT AGAR Na. Cl 7. 5% selective Mannitol differential Staphylococcus aureus can ferment mannitol, so the selective media used to isolate Staphylococcus aureus especially from nasal carriage is (Mannitol Salt Agar)
![Test S aureus Mannitol fermentation Positive S epidermidis Negative S saprophyticus Usually positive Positive Test S. aureus Mannitol fermentation Positive S. epidermidis Negative S. saprophyticus Usually positive Positive](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-10.jpg)
Test S. aureus Mannitol fermentation Positive S. epidermidis Negative S. saprophyticus Usually positive Positive = acid end products turn the phenol red p. H indicator from red to yellow negative = phenol red remains red Staphylococcus aureus turning the indicator from red to yellow
![Specimens 1 Direct Grams Slide 2 Biochemical Culture 4 3 Serology Antibiotics sensitivity 5 Specimens 1 Direct Gram’s Slide 2 Biochemical Culture 4 3 Serology Antibiotics sensitivity 5](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-11.jpg)
Specimens 1 Direct Gram’s Slide 2 Biochemical Culture 4 3 Serology Antibiotics sensitivity 5 6.
![Specimens Pus Swabs Urine C S F Sputum Blood In Cases Of Septicemia Specimens: Pus, Swabs Urine C. S. F. Sputum Blood In Cases Of Septicemia.](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-12.jpg)
Specimens: Pus, Swabs Urine C. S. F. Sputum Blood In Cases Of Septicemia.
![Biochemical tests Coagulase test DETECT the ability of S aureus to clot blood plasma Biochemical tests: Coagulase test: DETECT the ability of S. aureus to clot blood plasma](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-13.jpg)
Biochemical tests: Coagulase test: DETECT the ability of S. aureus to clot blood plasma (fibrinogen fibrin). Slide method Tube method Catalase test: To differentiate between Staph. & Strepto.
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![Coagulase test Coagulase test](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-15.jpg)
Coagulase test
![C o a gu l a s e t e s t ve ve C o a gu l a s e t e s t -ve +ve](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-16.jpg)
C o a gu l a s e t e s t -ve +ve -ve
![Catalase test Positive by bubble formation Catalase test Positive by bubble formation](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-17.jpg)
Catalase test Positive by bubble formation
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![Table summarized the point of differences between 3 imp Staphylococci species Tests S aureus Table summarized the point of differences between 3 imp. Staphylococci species Tests S. aureus](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-20.jpg)
Table summarized the point of differences between 3 imp. Staphylococci species Tests S. aureus S. epidermidis S. saprophyticus Catalase + + + Coagulase + - - β – haemolysis + - - Mannitol fermentation + - - Novobiocin sensitivity (0. 5 µg) + + -
![Gelatinase test Nutrient gelatin is a differential medium that tests the ability of an Gelatinase test Nutrient gelatin is a differential medium that tests the ability of an](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-21.jpg)
Gelatinase test Nutrient gelatin is a differential medium that tests the ability of an organism to produce an exoenzyme, called gelatinase that hydrolyzes gelatin. The gelatinase test can be used to differentiate between Staphylococcus aureus and Staphylococcus epidermidis. Test S. aureus Gelatina se Positive S. epidermidis Negative
![Summary Genus Staphylococci Gram positive cocci in shape There are 3 Summary: • Genus Staphylococci Gram positive , cocci in shape. • There are 3](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-22.jpg)
Summary: • Genus Staphylococci Gram positive , cocci in shape. • There are 3 medically important types. • We can differentiate between these types depending on culture characteristics also several biochemical tests. DR. SARMAD ZEINY, MBCh. B, MSc.
![THANKS THANKS](https://slidetodoc.com/presentation_image_h/096ba61cb9230f7022732e8a2a2be609/image-23.jpg)
THANKS
Novobiocin test
Coagulase negative staphylococcus
Curb-65
Cocci
Gram positive cocci in urethral discharge
Gram positive cocci
Gram positive cocci
Catalase test procedure
Positive catalase test
Oxidase test
Macconkey agar composition
Staph aureus morphology
Sarcina cocci
Qcm cocci gram positif
Catalase oxydase
Staphylococcus aureus cocci or bacilli
Coccobacilli
Rods or cocci
Catalase test
Oxidase results
Staphylococcus saprophyticus on blood agar
Elek
Divisão celular
Listeria catalase