Genomic DNA prep groups PM 1 2 3

Genomic DNA prep groups PM 1 2 3 4 5 6 7 8

PCR results, groups g is g. DNA C is c. DNA msp 2 msp 3 PM + - + Pf. CLAMP PM + + - - msp 4 +g +c - pfd 0100 c + + - eba 175 eba 181 + - + Pfs 16 - + -

RNA 1 x TBE gel A A: ring stage T: Troph stage E: Schizont stage T E RT-PCR with “K 2” oligo mix, “+” is with reverse transcriptase, “-” is without reverse transcriptase + A - + T - + E -

msp 2 PM T E msp 3 ct - T E msp 4 ct - T E ct - Gene specific PCR using c. DNAs T and E: msp 2, msp 3, msp 4, eba 175, Eba 181, pfs 16. Pfclamp on small gel eba 175 PM T E ct eba 181 - T E ct Pf. CLAMP Pfs 16 - T E ct - PM T E ct -

Bam. HI/Eco. R 1 digest of p. GEX clones msp 2 PM 1 2 3 msp 3 4 5 6 1 msp 4 c. DNA PM 1 2 3 4 5 6 eba 175 6 1 2 3 4 5 p. GEM-msp 4 genomic

Bam. HI/Eco. R 1 digest of p. GEX clones eba 181 PM? 1 2 3 Pfs 16 4 1 2 Pf. CLAMP PM 1 2 3 4 5 6

m 5 sp 1 Pf 19 s 1 6 Pf CL A GS MP T 17 eb a m sp 4 3 2 m sp sp m PM P M 81 6 A 1 L A fs 1 f. C B P E P 40 k. Da 30 k. Da 10% gel 26 k. Da All proteins are fused with GST (26. 6 k. Da). The MW standard on the right gel was Thermo’s Broad range prestained protein ladder 15% gel

7) 3 D Pf p s 1 M 6 SP 1 EB 19 A 1 75 M SP M 4 SP 3 M SP 2( lam GS T Pf C (you can cut out the part of the image you want to show; the original membranes are under the western pic)