Genome Editing Dieter Steinmetz ZMBP Universitt Tbingen Kurs

Genome Editing Dieter Steinmetz ZMBP Universität Tübingen Kurs SS 2018

Genome Editing ZFN „Zinc Finger Nuclease“ TALEN “Transcription Activator-Like Effector Nuclease” CRISPR/Cas 9 nuclease “Clustered Regularly Interspaced Short Palindromic Repeats/Cas 9 nuclease” ZMBP 1 2 3 Quelle: 1. Baltes & Voitas 2014 2. Wikipedia Commons 3. Doudna J. & Charpentier, E. Science 346(6213): 1077 2014

Recent advances enable precision in editing RNAi Mediated Gene Knockout Random Mutagenesis & Integration Targeted Integration via Homologous Recombination First Generation Genome Editing Tool Zinc Finger Technology

ZFN – „Zinc Finger Nuclease“ TALEN – “Transcription Activator-Like Effector Nuclease” CRISPR/Cas 9 nuclease – “Clustered Regularly Interspaced Short Palindromic Repeats/Cas 9 nuclease” ZMBP

ZFN – TALEN – CRISP – gleicher Mechanismus Suchsequenz DSB Disruption oder Substitution Chen et al. Giga. Science 2014 3: 24 doi: 10. 1186/2047 -217 X-3 -24 ZMBP

ZFN – TALEN – CRISP – Gene targeting efficiency Fig A Fig B Fig C Fig D Merkle FT et al. (2015). Efficient CRISPR-Cas 9 -Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus. Cell Reports. Volume 11, 6, 12 May 2015, Pages 875– 883 http: //www. sciencedirect. com/science/article/pii/S 2211124715003824 ZMBP

ZFN – TALEN – CRISP Quelle: Sabine Müller, ZMBP

Gene Editing – Future and society Technique CRISPR/Cas 9 perspectives in society. Human genetic engineering Quelle: J. Searle Dawley CC Green genetic engineering Quelle: David John Galvin, s. m. CC BY-SA 4. 0 Gene Drive – control of diseases ZMBP Quelle: National Library of Medicine CC

experiment Conclusions CRISPR/Cas 9 simple in lab Quelle: Magnus Manske CC BY Fast and precice experience Cheap Quelle: Sonja Longford CC 0 ZMBP Quelle: US Federal Government CC