Genetics and Development Embryology Genetics n How did

Genetics and Development

Embryology & Genetics n How did the work of Boveri and Stevens support the chromosomal hypothesis of inheritance?

Embryology & Genetics n In what way were Gluecksohn-Shoenheimer and Waddington early pioneers in developmental genetics?

Genomic Equivalence n What is meant by a totipotent nucleus?

Genomic Equivalence n n How were Briggs and King able to show that a blastula nucleus was totipotent? What if more advanced nuclei were used?

Genomic Equivalence

Genomic Equivalence n How does the potency of somatic cells from Rana pipens compare with those from Xenopus laevis? n How did Gurdon’s transplant technique differ from Briggs and King?

Genomic Equivalence n How was the first mammal cloned?

Genomic Equivalence

Differential Gene Expression n How do we know that only a small part of the genome is expresse in each cell?

Molecular Biology Techniques n How can northern blotting be used to obtain information about development?

Molecular Biology Techniques

Molecular Biology Techniques n What’s involved in reverse transcriptasepolymerase chain reaction (RT-PCR)?

Molecular Biology Techniques n Why is RT-PCR valuable in developmental studies?

Molecular Biology Techniques n For what are DNA microarrays used?

Molecular Biology Techniques n What is involved in in situ hybridization?

Molecular Biology Techniques Whole-mount in situ hybridization

Molecular Biology Techniques n Transgenic cells & organisms – What techniques are used to insert DNA into cells? n Microinjection n Transfection n Electroporation n Retroviral n. P element vector

Molecular Biology Techniques Producing chimeric mice

Molecular Biology Techniques n What’s the difference between transgenic and “knockout” mice?

Molecular Biology Techniques n What is antisense m. RNA and how may it be used?

Molecular Biology Techniques n What is RNA interference and how is it used?