Genetic Engineering Part 2 Applications of Recombinant DNA

Genetic Engineering Part 2 Applications of Recombinant DNA Pharmaceuticals Recombinant Vaccines

Describe what is happening at each number. 1 4 5 6 7 3 2 If you forgot watch this video again. https: //www. youtube. com/watch? v=LP 5 Tct. APPUI

Use Google or your textbook What is a transgenic organism? What is a vector (in genetic engineering)?

Genetically Modifying Bacteria to Produce Human Proteins Bacteria The gene for a human protein is cut of the chromosome Plasmid vector inserted into bacteria - (The gene for a human protein) The gene for a human protein Plasmids replicate and the bacteria produces the human protein

Pharmaceuticals (human proteins that people need) Produced Using Recombinant DNA insulin u human growth hormone u interferon u factor VIII u tissue plasminogen activator (TPA) u erythropoietin u

Protein u interferon u factor VIII u tissue plasminogen activator (TPA) u erythropoietin Function protects cells from viral infections; signals nearby cells to heighten their anti-viral defenses clotting factor (helps the blood form clots) breaks down blood clots promotes the production of red blood cells

What is the function of a vaccine?

The Cowpox virus was used as a vaccine against smallpox.

Harmless Cowpox Virus HIV (AIDS Virus) - (HIV envelope protein)

Why would you want to make a harmless virus look like HIV? Harmless Cowpox Virus w/HIV envelope protein

Scientists are working on a vaccine for AIDS. One group of genetic engineers would like to produce an AIDS vaccine by genetically engineering a harmless cowpox virus so that it produces the AIDS virus envelope protein (an outer covering around the HIV virus). How could this be done?

Making a Recombinant Vaccine 1. Genetic material from the AIDS virus is extracted from the virus. All the AIDS genes except the gene for making the virus envelope protein are destroyed. 2. The DNA from a harmless cowpox virus is extracted. 3. The DNA for the AIDS gene that makes the AIDS virus envelope protein is combined with the cowpox DNA. (4) The newly combined DNA is allowed to replicate, producing recombinant cowpox viruses inside AIDS virus envelopes. (5) The new viruses will be injected into a human being. 1 1 * 2 Gene for surface envelope protein 5 4 3

Making a Recombinant Vaccine 1. Why are all the genes of the AIDS virus destroyed except for the gene for making the viral envelope protein? 2. Why is the gene for making the viral envelope protein combined with cowpox virus DNA? 3. Why would this new virus be considered a vaccine? 4. Why, in the future, might this vaccine not be effective against HIV?

Making a Recombinant Vaccine For Hepatitis B 1. What is the goal of this procedure? 2. Why are they putting a gene from the Hepatitis B virus into yeast cells?

Scientists are currently trying to produce a recombinant vaccine for Covid- 19 Partnership for the development of a recombinant receptor-binding domain spike proteinbased severe acute respiratory syndrome coronavirus vaccine. c. GMP: Current good manufacturing practice.