GENETIC ENGINEERING Genetic Engineering aka Genetic Modification Make

GENETIC ENGINEERING

Genetic Engineering aka . . Genetic Modification Make changes in DNA code by Insert or delete specific genes Use modern molecular biology techniques.

1) Recombinant DNA Aka. . Gene Splicing made by combining DNA from > 1 source (often from very different species)

2) Gel electrophoresis An electric current smaller fragments faster larger ones – slower DNA fragments move to positive end + - A B DNA on murder weapon

3)Transgenic organisms carries a foreign gene, deliberately inserted into its genome. transgenic animal Mice--- so immune sys more like humans- can study ‘em Livestock --- leaner meat transgenic plant Soybeans/corn/crops --- to produce nat insecticiside so don’t have to be sprayed Also: Improved Nutritional Quality Insect Resistance Disease Resistance Drought Tolerance

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4) Cloning Clone --- genetically identical copy replacing the nucleus of an unfertilized ovum with the nucleus of a body cell from the organism. first adult mammal cloned was Dolly in 1997

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5) DNA Fingerprinting Compare genetic similarities/differences b/w individuals. often used to ID source of blood / tissue found at crime scenes. Polymerase Chain Reaction (PCR)

6) Gene Therapy the insertion of normal or genetically altered genes into cells usually to replace defective genes (cancer & genetic disorders) Eye cancer

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My lab experience…….

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