General Microbiology Laboratory Bacterial Generation Time Microbial Growth

General Microbiology Laboratory Bacterial Generation Time

Microbial Growth v Growth: an increase in the number of cells, not an increase in size v Bacterial species only maintained if population continues to grow v Generation: growth by binary fission v Growth rate: cell number/time or cell mass/time v Generation time: time it takes for a cell to divide and the population to double; most are 1 -3 hours. v Generation times vary markedly with the species of microorganism and environmental conditions; they can range from 10 minutes for a few bacteria to several days with some eucaryotic microorganisms.

Generation Times Generation Time Bacterium Medium (minutes) Escherichia coli Glucose-salts 17 Bacillus megaterium Sucrose-salts 25 Streptococcus lactis Milk 26 Streptococcus lactis Lactose broth 48 Staphylococcus Heart infusion broth 27 -30 aureus Lactobacillus Milk 66 -87 acidophilus Rhizobium Mannitol-salts-yeast 344 -461 japonicum extract Mycobacterium Synthetic 792 -932 tuberculosis Treponema pallidum Rabbit testes 1980

What occurs during binary fission?

The Growth Cycle Ø The population growth is studied by analyzing the growth curve of a microbial culture. Ø The standard bacterial growth curve describes various stages of growth a pure culture of bacteria will go through, beginning with the addition of cells to sterile media and ending with the death of all of the cells present.

Typical growth curve for a bacterial population

Phases of Growth

Lag phase • The period of apparent inactivity in which the cells are adapting to a new environment and preparing for reproductive growth. • Cells are usually synthesizing new components. • In practice, bacteria from one medium to another, where there are chemical differences between the two media, typically results in a lag in cell division. • This lag in division is associated with a physiological adaptation to the new environment. • Cells may increase in size during this time, but simply do not divide (by binary fission). • Lag phase varies considerably in length depending upon the condition of the microorganisms and the nature of the medium.

Log (exponential) phase • The period in which the organisms are growing at the maximal rate possible given their genetic potential, the nature of the medium, and the conditions under which they are growing. • Generation time can be easily obtained from the exponential phase of a growth curve • The population is most uniform in terms of chemical and physical properties during this period.

Stationary phase • Eventually population growth decrease, and the growth curve becomes horizontal. • rate of cell growth = rate of cell death • Cell death may result from Nutrient limitation & Toxic waste accumulation (e. g. acid buildup from fermentation); as well as O 2 depletion, critical population level reached.

Death phase • Stationary phase, in a standard bacterial growth curve, is followed by a die-off of cells, called Death phase. • It is the period in which the cells are dying at an exponential rate. • Some of the reasons are: continued accumulation of wastes, loss of cell's ability to detoxify toxins, etc.

End of lecture