GENE organism 1 organism 2 GENE organism 1

GENE organism 1 organism 2

GENE organism 1 organism 2

GENE coding segment 1 coding segment 2 coding segment 3 coding segment 4 coding segment 5 coding segment 6 isoform 1 isoform 2 coding segment 1 3 segment 2 isoform coding segment 3 coding segment 4 … coding segment n-1 coding segment n isoform 4 exon 1 exon 2 exon 3 … exon 4 isoform 1 isoform 2 … isoform k-1 exon m-1 isoform k exon m

Dme, Dps Aga

Dme, Dps Aga

Dme Dps Aga

stop Dme, Dps Aga (combined with acceptor site)

Dme, Dps Aga i ii iv

Evolution of the exon-intron structure and alternative splicing in fruit flies and malarial mosquito genomes D. B. Malko, V. J. Makeev, A. A. Mironov, M. S. Gelfand Genome Research, 2006 Apr; 16(4): 505 -509. Epub 2006 Mar 6.