Gene Editing Dieter Steinmetz Universitt Tbingen ZMBP 18

Gene Editing Dieter Steinmetz Universität Tübingen – ZMBP 18. 12. 15 Kurs WS 15/16

Gene Editing – 3 Main Methods ZFN „Zinc Finger Nuclease“ TALEN “Transcription Activator-Like Effector Nuclease” CRISPR/Cas 9 nuclease “Clustered Regularly Interspaced Short Palindromic Repeats/Cas 9 nuclease” ZMBP - Dieter Steinmetz Quelle: 1. Baltes & Voitas 2014 2. und 3. Wikimedia Commons

ZFN „Zinc Finger Nuclease“ ZMBP - Dieter Steinmetz

ZFN – TALEN – CRISPR – gleicher Mechanismus Suchsequenz DSB Disruption oder Substitution Chen et al. Giga. Science 2014 3: 24 doi: 10. 1186/2047 -217 X-3 -24 ZMBP

Gene targeting effektiver in CRISPR Gene targeting Experimente Merkle FT et al. (2015). Efficient CRISPR-Cas 9 -Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus. Cell Reports. Volume 11, 6, 12 May 2015, Pages 875– 883 http: //www. sciencedirect. com/science/article/pii/S 2211124715003824 ZMBP

Protein B Protein A

Recent advances enable precision in editing Random Mutagenesis & Integration RNAi Mediated Gene Knockout Targeted Integration via Homologous Recombination First Generation Genome Editing Tool Zinc Finger Technology

Recent advances enable precision in editing RNAi Mediated Gene Knockout Random Mutagenesis & Integration Targeted Integration via Homologous Recombination First Generation Genome Editing Tool Zinc Finger Technology

Random Mutagenesis RNAi Mediated Gene Knockout & Integration Targeted Integration via Homologous Recombination First Generation Genome Editing Tool Zinc Finger Technology