Gel Electrophoresis Student Instructions Edvotek set up Salk

Gel Electrophoresis Student Instructions Edvotek set up Salk Institute Mobile Lab

Gel Electrophoresis Student Station Set-up tip container w/tips waste container micropipettor foam epi rack w/ 1 food color tube each: orange, green, purple Salk Institute Mobile Lab practice sheet in protector

How to Use a micro-pipettor Plunger • Parts of a Micro-pipettor – Make sure a plastic tip is firmly twisted onto the end of the micro-pipettor – Adjust the dispense amount to 5 µl (05. 0) by turning the knob at the top Eject button Amount window Barrel Plastic tip Salk Institute Mobile Lab

How to Use a micro-pipettor • Carefully open one of the food color tubes – Don’t let the lid snap back and splash you! • To Fill – press down gently with your thumb until you feel the plunger stop. This is STOP 1. While holding the plunger at Stop 1 place the tip into the liquid and release the plunger. This will draw up the precise volume you want. Salk Institute Mobile Lab

How to Use a micro-pipettor • To Dispense – press down hard on the plunger, you’ll pass Stop 1 and continue to STOP 2. This will fully eject the volume from the tip. • Using your other hand to guide the end of the tip, practice dispensing the 5µl drops – Try to not push down on the plastic sheet - see if you can hold the pipettor tip just above the paper Salk Institute Mobile Lab

How to Use a micro-pipettor • Push ejector button to eject the dirty tip into the waste container and replace with a clean tip • Always use a clean tip each time!! Salk Institute Mobile Lab

How to Use a micro-pipettor • Ready to load a real gel? Salk Institute Mobile Lab

Loading the gel • Load the food color solutions into the wells on your group’s gel • Be careful not to poke through the bottom of the wells! Salk Institute Mobile Lab