GdDTPA as an MRI Contrast Agent Lindsay Dods

Introduction ¡ MRI – Magnetic Resonance Imaging l ¡ Contrast Agents l ¡ Can

Objectives Show the effectiveness of Gd-DTPA (Magnevist) as a contrast agent in high field

Approach ¡ Compare contrast in mice brains l l ¡ before and after incubation

Hypothesis The mean signal intensity of the brain will increase with the addition of

Methods ¡ Prepare Samples l l Split fixed brain into two hemispheres Incubate one

Methods ¡ Scan l Perform T 1 and T 2 waited scans with a

Results ¡ Using Regression Analysis, P<0. 05, therefore result is significant.

Discussion Results aren’t as expected ¡ Paramagnetic contrast agents alter signal intensity by shortening

Future Work Contrast agent in disease diagnosis ¡ Use this protocol on novel contrast

Conclusion Results were not conclusive to show that signal intensity increased over time with

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Gd-DTPA as an MRI Contrast Agent Lindsay Dods April 6, 2010 Supervisors: Robert Ta, Masters Student Robert Bartha, Ph. D.

Introduction ¡ MRI – Magnetic Resonance Imaging l ¡ Contrast Agents l ¡ Can be used to detect anatomical structures and function. Can enhance the contrast of an MRI image, allowing for increased differentiation. Novel Contrast Agents must be tested

Objectives Show the effectiveness of Gd-DTPA (Magnevist) as a contrast agent in high field MRI. ¡ As this agent is known to be effective, the true objective is to assess the testing protocol before using novel contrast agents. ¡

Approach ¡ Compare contrast in mice brains l l ¡ before and after incubation in contrast agent different incubation times. Contrast is ratio of mean signal intensity of the brain compared to control

Hypothesis The mean signal intensity of the brain will increase with the addition of contrast agent ¡ This signal intensity will continue to increase over time, until a saturation point is reached ¡ Intensity of the control is to remain constant. ¡

Methods ¡ Prepare Samples l l Split fixed brain into two hemispheres Incubate one side of the brain in diluted Gd-DTPA, use other side as control

Methods ¡ Scan l Perform T 1 and T 2 waited scans with a 9. 4 T MRI. Measure mean grey level of each hemisphere from select slices in Image. J ¡ Calculate Contrast ¡

Results ¡ Day 1, T 2 ¡ Day 6, T 2

Results ¡ Using Regression Analysis, P<0. 05, therefore result is significant.

Discussion Results aren’t as expected ¡ Paramagnetic contrast agents alter signal intensity by shortening relaxation time of protons ¡ Increase in signal intensity is beneficial ¡

Future Work Contrast agent in disease diagnosis ¡ Use this protocol on novel contrast agents ¡

Conclusion Results were not conclusive to show that signal intensity increased over time with the addition of a contrast agent. ¡ More samples are needed to determine if this protocol is an effective way to test contrast agents. ¡