GCL A NBL ED 18 5 PND 3

GCL A NBL ED 18. 5 PND 3 PND 6 B GCL INL ONL PND 60

A B GS CDH 11 Merge 40 x oil Chx-10 Cralbp CDH 11 Merge 100 x oil C 160 k. Da CDH 11 Merge 40 x oil D HPC-1 CDH 11 40 x oil Chx-10 Merge 40 x oil 100 x oil CDH 11

E 18. 5 A B C P 3 P 6 P 15 P 60

CDH 11+/+ ED 18. 5 A CDH 11 -/- PND 3 CDH 11+/+ CDH 11 -/- PND 6 CDH 11+/+ Chx 10 160 k. Da HPC B Brd. U CDH 11+/+ CDH 11 -/- C NCDH ED 18. 5 PND 3 PND 6 CDH 11+/+ CDH 11 -/-

Image J quantification

TAg+/- ; CDH 11+/+ TAg+/- ; CDH 11+/- TAg+/-; CDH 11 -/- TAg+/- ; CDH 11+/+ B TAg+/- ; CDH 11+/- TAg+/-; CDH 11 -/- Total # of T-Antigen positive cells in retina/eye Mouse 1 81 57 43 Mouse 2 132 52 19 Mouse 3 71 72 29 Mouse 4 69 83 40 Mouse 5 92 34 46 Avg: 89 66 33 C Tag+ve cells / retina [cells/pixelsx 10^-4] A 10 K-W Test: p=0. 0078 9 8 7 6 5 4 3 2 1 0 TAg+/; CDH 11+/+ TAg+/; CDH 11+/Genotype TAg+/; CDH 11 -/-

TAg+/- ; CDH 11+/+ A B TAg+/-; CDH 11 -/- TAg+/- ; CDH 11+/- % Tumor/retina [pixles] Percent tumors in retinas of mice at PND 28 9 8 7 6 5 4 3 2 1 0 TAg+/- ; CDH 11+/+ TAg+/- ; CDH 11+/Genotype K-W Test: p=0. 0167 TAg+/-; CDH 11 -/-

TAg+/- ; CDH 11+/+ A B % Tumor per retina 30 TAg+/-; CDH 11 -/- TAg+/- ; CDH 11+/- Percent tumors in retinas of mice at PND 84 K-W Test p=0. 0602 25 20 15 10 5 0 TAg+/- ; CDH 11+/+ TAg+/- ; CDH 11+/Genotype TAg+/-; CDH 11 -/-

Antibody Name Company Dilution Used SV 40 Tag (Pab 101) mouse monoclonal Santa Cruz Biotechnology Cat# SC-147, Lot# A 2506, 1: 200 CDH 11 - clone CDH 113 H mouse monoclonal From St. John at ICOS Corp. 1: 2, 500 CDH 2 (N-cadherin) mouse monoclonal BD Biosciences Pharmigen Cat# 610920, Lot# 06247 1: 2000 Brd-U (purified antibromodeoxyuridine) mouse monoclonal BD Biosciences Pharmigen Cat# 555627, Lot#52817, 1: 200 Progenitors and Bipolars: Chx 10 sheep polycolonal gift from Rod Bremner, UHN 1: 1000 Apoptosis: activated caspase-3: anti-h/m Caspase 3 (active) rabbit polyclonal R&D Systems Cat# AF 835, Lot# CFZ 326011 1: 500 Early Müller: Hes-5 rabbit polyclonal gift from John Saari 1: 2000 Müller: Vimentin goat polyclonal Santa Cruz Biotechnology Cat# Lot# 1: 100 Ganglion - Brn 3 b goat polyclonal Sant Cruz Biotechnology Cat# sc-6026 1: 100 Amacrine – Syntaxin clone HPC-1 mouse monoclonal Sigma Cat# S 0664 1: 200 Horizontal – 160 k. Da mouse monoclonal Sigma 1: 40

Staining for Cralbp, Muller cell marker 6 litters analyzed Birth: P 3 CDH 11+/+ P 6 NBL CDH 11 -/-

CDH 11 is gradually lost in tumors of Tag-RB mice Anti-SV 40 lg(Tag) 4 wks Tag-RB 129/C 57 Bl-6 5 mth Tag-RB C 57 Bl-6 Anti-CDH 11