GBBE Intro To Microorganisms Equipment 4 Flasks 1

GBBE: Intro To Microorganisms

Equipment ● ● ● ● ● 4 Flasks 1 stopper 2 stopper with hole 1 bent glass hot plate broth (2. 4 g) 500 m. L Autoclave Jar 100 m. L Graduated Cylinder Water

Procedure 1. Prepare a solution of broth in an autoclave jar ● use 300 ml of water and 2. 4 g of powder nutrient broth ● mix until powder is dissolved 2. Divide the solution between four flasks ● Each should have 75 ml 3. Heat up each flask with the broth in it until the broth reaches a boil ● let flasks cool 4. Cover flasks with stoppers ● place stopper (no hole) over Flask 1 ● place stopper (with hole) over Flask 2 ● place stopper (with hole) over Flask 3 and place the bent glass in the stopper ● leave Flask 4 uncovered 5. Observe flasks over the course of a week and record all necessary data.

Results: Flask One ● ● ● Day one- no growth Day two- no growth Day three- no mold and cloudy Day four- cloudy Day five- cloudy

Flask One:

Results: Flask Two ● ● ● Day one- no growth Day two- no growth Day three- bits of mold and cloudy Day four- bits of mold Day Five- bits of mold

Flask Two:

Results: Flask Three ● ● ● Day one- no growth Day two- no growth Day three- cloudy and mold on surface Day four- cloudy and mold on surface Day five- cloudy, mold on surface, and bits of mold

Flask Three:

Results: Flask Four ● ● ● Day one- no growth Day two- cloudy Day three- evident growth (cloudy and moldy) Day four- cloudy and moldy Day five- mold on surface and large bits of mold

Flask Four: