Gas Chromatography Flame Ionization Detector or GCFID Gas

Gas Chromatography – Flame Ionization Detector or GCFID Gas chromatography is a technique for separating chemical substances that relies on differences in partitioning behavior between a flowing mobile phase and a stationary phase to separate the components in a mixture. The flame ionization detector (FID) is the most sensitive gas chromatographic detector for hydrocarbons such as butane or hexane. Principle of gas chromatography: The sample solution injected into the instrument enters a gas stream which transports the sample into a separation tube known as the "column. " (Helium or nitrogen is used as the so-called carrier gas. ) The various components are separated inside the column. The detector measures the quantity of the components that exit the column. To measure a sample with an unknown concentration, a standard sample with known concentration is injected into the instrument. The standard sample peak retention time (appearance time) and area are compared to the test sample to calculate the concentration.

GC Columns & Consumables SAMPLE INTRODUCTION-Syringes SAMPLE INTRODUCTION-Septa SAMPLE INTRODUCTION-Liners SEPARATION-GC Connections (Ferrules and Unions) SEPARATION-GC Capillary Columns

List of Capillary Column Phase Applications Polarity Temperature range °C HP-FFAP (Nitroterephthalic acid modified polyethylene glycol) (0. 52 um, 0. 32 mm I. D. X 25 m long) Acids, Alcohols, Aldehydes, Acrylates, Ketones, Nitriles High polarity 60 – 240/250 °C Inno-Wax (polyethylene glycol (PEG) (0. 5 um, 0. 32 mm I. D. X 30 m long) Alcohols, Free Acids, Aromatics, Essential Oils, Solvents High polarity -20 – 260 °C HP-1 (Cross linked Methyl Siloxane) (0. 88 um, 0. 53 mm I. D. X 5 m long) hydrocarbons, pesticides, polychlorinated biphenyls (PCBs), and sulfur compounds. non polar ° 325/350 – 60 - HP-5 (5%-Phenyl)-methylpolysiloxane) (0. 1 um, 0. 1 mm I. D. X 10 m long) Alkaloids, drugs, FAMEs, halogenated compounds, aromatic compounds non polar ° 325/350 – 60 - DB-Wax (Polyethylene glycol (PEG) (0. 25 um, 0. 32 mm I. D. X 30 m long) Alcohols, glycols, aromatics polar 40 -240 °C

GC Column Phase Selection Guide

Features of HP-1 Column

HP-5