Garlic Armageddon Bio Sat Lauren Persons Ryder Whitmire

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Garlic Armageddon Bio. Sat Lauren Persons, Ryder Whitmire, Lenny Komow, Kirstyn Johnson, Jon Pfohl,

Garlic Armageddon Bio. Sat Lauren Persons, Ryder Whitmire, Lenny Komow, Kirstyn Johnson, Jon Pfohl, Dave Borncamp October 6 th 2009

Mission Overview Mission Statement - Analyze effect of radiation and atmosphere on growth rate

Mission Overview Mission Statement - Analyze effect of radiation and atmosphere on growth rate of bacteria Mission Objectives - Send bacteria up with Geiger counter, pressure sensor, temperature sensor, heater - Compare bacteria to control ground group (quantitatively and spectrophotometrically) Mission Expectations - Bacterial recovery rates = low compared to control - - Radiation, (heat can be a factor) Implications - Accidentally carrying bacteria into space

Requirements Flow Down • 2. 1 Level 0 Requirements: 1. Weigh less than 850

Requirements Flow Down • 2. 1 Level 0 Requirements: 1. Weigh less than 850 grams, budget of $100 - contains Geiger counter, Petri dish and tube (with E. coli bacteria) 2. Measure amounts of radiation in near space, the effects it has on the bacteria - fulfill mission objective of documenting survival rate of bacteria exposed to radiation • 2. 2 Level 1 Requirements: 1. Geiger counter = counts of radiation as function of altitude 2. Petri dish and tube of e. coli placed in front of glass window and exposed to radiation from atmosphere 3. Temperature sensor measures how much impact temperature has on the e. coli

Design • Two plates of bacteria: 1) will be a control 2) will be

Design • Two plates of bacteria: 1) will be a control 2) will be exposed to radiation of upper atmosphere - The plates test quantifiable data • Tubes of bacteria (in broth) - Test spectrophotometrically • How it will be recorded - Radiation recorded by Geiger counter every 5 seconds (pressure recorded too) - Will be used to plot radiation as a function of altitude

Parts List Satellite Parts Geiger counter Petri dish Agar Nutrient broth Tube + cap

Parts List Satellite Parts Geiger counter Petri dish Agar Nutrient broth Tube + cap Glass filter Bacteria Batteries Dry Ice Polyolefin film Insulation HOBO Datalogger AVR Board Camera Heater

Diagram

Diagram

Functional Block Diagram Power Heater Camera Power Switch 3 (Batteries) AVR Controller Petri Temp

Functional Block Diagram Power Heater Camera Power Switch 3 (Batteries) AVR Controller Petri Temp Sensor 1 Power Geiger Counter Power Internal Temperature Switch HOBO datalogger External Temperature Humidity

Weight Budget Component Weight (g) Geiger Counter 102 Foam. Core 75 Petri Dish 25

Weight Budget Component Weight (g) Geiger Counter 102 Foam. Core 75 Petri Dish 25 Pressure tube 15. 2 Pressure tube teflon cap OR Pressure tube nylon cap* 13. 2 3. 9 Geiger counter battery 40 Structure 25 Camera filter 30 Total 325. 4

Costs Material/Device Cost Source Batteries $20 Grocery Store Geiger Counter Donated Images Co. Polyolefin

Costs Material/Device Cost Source Batteries $20 Grocery Store Geiger Counter Donated Images Co. Polyolefin film Donated Europack Inc. Petri Dishes Donated Biology Dept at CU Bacteria Donated Carolina Biological Temperature Sensor $5 Electronics Center, ITLL Agar Donated MCDB Dry Ice $20 Grocery Store Pressure tube Donated Ace Glass Inc. Insulation $10 Home Depot Camera filter Donated B&H Photo Video Total 65

Schedule Date Tasks 9/22/09 9/29/09 Conceptual Design Review Prototyping design complete Authority to Proceed

Schedule Date Tasks 9/22/09 9/29/09 Conceptual Design Review Prototyping design complete Authority to Proceed and Hardware Ordering 10/1/09 Design complete Order or acquire all hardware Completion of Vacuum Test Design Document Revisions A/B Critical Design Review Testing final structure Complete Cold test Completion of Geiger counter/ Radiation Test Completion of Board and Sensors Test 10/6/09 10/15/09 10/22/09 10/27/09 10/29/09 10/31/09 11/3/09 11/5/09 11/6/09 11/7/09 11/8/09 12/1/09 12/5/09 12/8/09 Software Test In Class Mission Simulation Test Grow cultures both as control and for flight Design Document Revision C Launch Readiness Review Pre-launch Testing of Bacteria Completed Balloon. Sat Weigh-in and Turn in Launch Day Post-launch Testing of Bacteria Completed Design Document Revision D Final Presentation Design Expo Balloon. Sat Hardware Turn In

Proposal Requirements • The Geiger counter does not have an output that exceeds five

Proposal Requirements • The Geiger counter does not have an output that exceeds five volts • The heater and insulation will keep the internal temperature above -10 degrees C • The combined components will not exceed 850 grams (if it does exceed, modifications will need to be made) • The team will maintain a safe environment, and take precautions to protect the electrical components

Testing • Structural Tests: Drop Test, Whip Test, Cold Test – Completed by 10/15/09

Testing • Structural Tests: Drop Test, Whip Test, Cold Test – Completed by 10/15/09 • Scientific Tests: Pressure and Temperature Testing – Bacterial Response – Geiger Counter – Pressure Tubes – Completed by 10/22/09 • Software Testing – Geiger Counter – Camera – Completed by 10/22/09

Expected Results • Cell death by radiation – Decreased colony growth • Replica Plating

Expected Results • Cell death by radiation – Decreased colony growth • Replica Plating – Slower propagation in nutrient broth • Spectrophotometric Analysis

Organizational Chart Project Management: Kirstyn Johnson, lead Ryder Whitmire, assistant Structures: David Borncamp, lead

Organizational Chart Project Management: Kirstyn Johnson, lead Ryder Whitmire, assistant Structures: David Borncamp, lead Leonard Komow, assistant Systems Management: Scientific: Ryder Whitmire, lead Jonathan Pfohl, assistant Kirstyn Johnson, assistant Electrical: Programming: Lauren Persons, lead Leonard Komow, lead David Borncamp, assistant Lauren Persons, assistant

Biggest Worries • • Preventing bacterial death Agar in a vacuum Building the Geiger

Biggest Worries • • Preventing bacterial death Agar in a vacuum Building the Geiger counter Weight