Fun with Volume Measurement Ellyn Daugherty SM Biotech

Fun with Volume Measurement Ellyn Daugherty SM Biotech Career Pathway www. Biotech. Ed. com www. emcp. com/biotech Ellyn@Biotech. Ed. com www. sargentwelch. com/biotech • • Text - Chapter 3 Lab Manual - Chapter 3 Student CD Tutorials Course Planner

Volume Measurement in Biotech 1 Chapter 3

Start with Graduated Cylinders On all instruments: • What is the total volume that can be measured? • What is the value of each type of graduation?

Review: Metric Measurements and Conversions • L 1000 m. L 92 m. L = _____ µL 0. 5 L = _____ m. L 1000 µL 357 µL = _____ m. L 0. 557 m. L = _____ µL

Measuring Small Volumes with Pipets • Start with 10 -m. L pipets • Show it can be read up or down • Show zero, then to tip • Explain “TD” • Demo green pump and drawing up and dispensing • Add 5. 60 m. L of red solution to tube #1 • Add 2. 80 m. L of red solution and 2. 80 m. L H 20 to tube #2, cap, mix • Compare tubes with other techs • Use the spec (at 400 nm) to compare them

Measuring Small Volumes with Pipets • Repeat with 5 -m. L pipets • Show it can be read up or down • Show zero, then to tip • Use green pump • Using 13 x 100 mm, add 3. 60 m. L of red solution to tube #3 • Add 1. 80 m. L of red solution and 1. 80 m. L H 20 to tube #4, cap, mix • Compare tubes with other techs • Use the spec (at 400 nm) to compare them

Measuring Small Volumes with Pipets • Repeat with 2 -m. L pipets • Show it can be read up or down • Show zero, then to tip • Use blue pump • Using 13 x 100 mm, add 1. 90 m. L of red solution and 1. 90 m. L H 20 to tube #5, cap, mix • Using 13 x 100 mm, add 0. 95 m. L of red solution and 2. 85 m. L H 20 to tube #5, cap, mix • Compare tubes with other techs • Use the spec (at 400 nm) to compare them

Measuring Small Volumes with Pipets • Repeat with 1 -m. L pipets • Show it can be read up or down • Show zero, then to tip • Use blue pump • Using 13 x 100 mm, add 0. 90 m. L of red solution and 2. 70 m. L H 20 to tube #5, cap, mix • Using 13 x 100 mm, add 0. 45 m. L of red solution and 3. 35 m. L H 20 to tube #5, cap, mix • Compare tubes with other techs • Use the spec (at 400 nm) to compare them

Measuring Very Small Volumes with Micropipets • Start with any of the micropipets • Show to determine its size and range • Show to hold it so display can be read. • Demonstrate how to read it • Demonstrate first and second stop • Demonstrate how to put a tip on and eject the tip. • Demonstrate how to take up and dispense a sample.

Measuring Very Small Volumes with Micropipets • Using the P-100 and yellow tips • Each partner measures 75. 2 µL of one dye into their own 1. 7 m. L tube. • Demonstrate how to compare tube • Demonstrate to check it by sucking it up

Measuring Very Small Volumes with Micropipets • Using the P-10 and white tips • Each partner measures 9. 85 µL of one dye into their own 1. 7 m. L tube. • Demonstrate how to compare tube • Demonstrate to check it by sucking it up

Measuring Very Small Volumes with Micropipets • Using the P-1000 and blue tips • Each partner measures 333 µL of one dye into their own 1. 7 m. L tube. • Demonstrate how to compare tube • Demonstrate to check it by sucking it up

Micropipeting Secret Code

Get help starting or improving a biotech program ! Ellyn Daugherty Amy Naum SM Biotech Career Pathway www. SMBiotech. com www. Biotech. Ed. com www. emcp. com/biotech www. sargentwelch. com/biotech Ellyn@Biotech. Ed. com VWR Ed/Sargent Welch www. sargentwelch. com/biotech Amy_Naum@vwreducation. com 585 -321 -9422 Holly Ahern VWR Ed/Sargent Welch www. sargentwelch. com/biotech Holly_ahern@vwreducation. com 512 -789 -3155