Fruit Cup DNA Extraction Step 1 Into one

Step 1 • Into one of the 5 oz cups add 1 teaspoon of

Step 2 • Add 2 pinches of table salt. Fruit Cup DNA Extraction

Step 3 • Add 25 ml of distilled water to make a final volume

Step 4 • Using the plastic knife, peel and cut 1/2 of a kiwi

Step 5 • Using the plastic spoon, add the kiwi to the solution from

Step 6 • Mash the kiwi against the side of the cup with the

Step 7 • While one member of your group mashes the kiwi, another member

Step 7 (cont. ) • Fold the coffee filter’s edge around the cup so

Step 8 • Filter the mixture by pouring it into the filter and letting

Step 9 • Obtain a test tube of cold alcohol. For best results, the

Step 10 • Fill the plastic pipette with kiwi solution to the 1 ml

Step 11 • Add kiwi solution from the pipette to the cold alcohol. Fruit

Step 12 • Let the solution sit for 2 to 3 minutes without disturbing

Step 13 • You can watch the white DNA precipitate out into the alcohol

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Fruit Cup DNA Extraction

Step 1 • Into one of the 5 oz cups add 1 teaspoon of shampoo. Fruit Cup DNA Extraction

Step 2 • Add 2 pinches of table salt. Fruit Cup DNA Extraction

Step 3 • Add 25 ml of distilled water to make a final volume of 30 ml or about 1/3 the volume of the cup. • Dissolve the salt and shampoo by stirring slowly to avoid foaming. Fruit Cup DNA Extraction

Step 4 • Using the plastic knife, peel and cut 1/2 of a kiwi into small pieces. Fruit Cup DNA Extraction

Step 5 • Using the plastic spoon, add the kiwi to the solution from steps 1 -3. Fruit Cup DNA Extraction

Step 6 • Mash the kiwi against the side of the cup with the back of the spoon for 10 minutes. Fruit Cup DNA Extraction

Step 7 • While one member of your group mashes the kiwi, another member will place a #2 cone coffee filter inside the second 5 oz plastic cup. Fruit Cup DNA Extraction

Step 7 (cont. ) • Fold the coffee filter’s edge around the cup so that the filter does not touch the bottom of the cup. Fruit Cup DNA Extraction

Step 8 • Filter the mixture by pouring it into the filter and letting the solution drain for several minutes until there is approximately 5 ml (covers the bottom of the cup) of filtrate to test. Fruit Cup DNA Extraction

Step 9 • Obtain a test tube of cold alcohol. For best results, the alcohol should be as cold as possible. Fruit Cup DNA Extraction

Step 10 • Fill the plastic pipette with kiwi solution to the 1 ml mark. Fruit Cup DNA Extraction

Step 11 • Add kiwi solution from the pipette to the cold alcohol. Fruit Cup DNA Extraction

Step 12 • Let the solution sit for 2 to 3 minutes without disturbing it. • It is important not to shake the test tube. Fruit Cup DNA Extraction

Step 13 • You can watch the white DNA precipitate out into the alcohol layer. • DNA has the appearance of white, stringy mucus. Fruit Cup DNA Extraction