FREECLIMB Grape Gabriella De Lorenzis gabriella delorenzisunimi it

FREECLIMB Grape Gabriella De Lorenzis gabriella. delorenzis@unimi. it Rania Ahmed raaniaamed@yooh. it Kick-off meeting – Meknes, April 1 st-2 nd 2019

Partners involved UMIL: Università degli Studi di Milano (Italy) ARC: Agricultural Research Center (Egypt) Kick-off meeting – Meknes, April 1 st-2 nd 2019

Key ideotypes in Grape Biotic stress Downy mildew (Plasmopara viticola) resistance Abiotic stress Drought resistance - Salinity resistance Kick-off meeting – Meknes, April 1 st-2 nd 2019

Available plant materials Partner Biotic stress Abiotic stress UMIL Self-pollinated Mgaloblishvili population (116 genotypes). Mgaloblishvili is a vinifera Georgian variety resistant to downy mildew. M 1 x Vitis berlandieri (cultivar Thyers) population (164 genotypes). Caucasian grapevine collection (108 genotypes) for resistant traits againt powdery and downy mildew. ARC M 1 is a salinity resistant rootstock. V. berlandieri is a drought resistant species. Egyptian rootstocks resistant to salinity. Kick-off meeting – Meknes, April 1 st-2 nd 2019

WP 1: Phenotyping for traits relevant to resilient fruit system production Task 1. 1 Evaluation of plant response to biotic and abiotic stresses in germplasm collection Partner Biotic stress Abiotic stress UMIL Scoring for disease severity after artificial inoculation (Toffolatti et al. 2016, 2018) for downy mildew resistant traits. Greenhouse evaluation and field evaluation (not the first year). Leaf/canopy temperature with high-resolution thermal camera (Bianchi et al. 2019) for water stress resistance. Greenhouse evaluation at different soil water content from 80 to 20%. ARC In vitro evaluation of salinity resistance, measuring at different concentrations of sodium chloride, # of dead plants, plant size, root development and mineral composition will be determined by a flame photometer and/or ICP-MS. Kick-off meeting – Meknes, April 1 st-2 nd 2019

WP 2 activities: Genetic dissection and methods for selection of relevant traits for resistance to biotic and abiotic stresses Task 2. 1 Genome-wide genotyping and genetic characterization of germplasm resources Partner Biotic stress Abiotic stress UMIL Genotyping protocol: Vitis 18 k. SNP chip array (Laucou et al. 2018). ARC Microsatellites. Task 2. 2 Linkage and Association mapping • UMIL: QTL mapping for resistance to downy mildew. Kick-off meeting – Meknes, April 1 st-2 nd 2019

WP 3 activities: Exploitation of local biodiversity and development of pre-breeding materials Task 3. 1 Development of pre-breeding materials to pyramid resilience traits Partner Biotic stress Abiotic stress UMIL Pyramidized introgression in pre-breeding materials of powdery and downy mildew resistant traits. Introgression in vinifera germplasm drought and salt resistant traits, and phylloxera as well, coming from non-vinifera germplasm. ARC Task 3. 2 Setting up collections in multiple environments Partner Biotic stress UMIL A selection of the most interested genotypes will be shared between the two partners to be tested in different environments. ARC Abiotic stress Kick-off meeting – Meknes, April 1 st-2 nd 2019

WP 4 activities: Communication, dissemination, training and demonstration Task 4. 1 Database Phenotypic and genetic data will be included in FREECLIMB database. Kick-off meeting – Meknes, April 1 st-2 nd 2019

Programmed activities: first year WP 1 - Task 1. 2 Phenotyping segregating progenies to decipher genetic determinism for traits relevant to predefined ideotypes Partner Biotic stress Abiotic stress UMIL Phenotyping Mgaloblishvili progeny (116 genotypes) for resistance against downy mildew. Phenotyping M 1 x V. berlandieri progeny (164 genotypes) for drought resistance. ARC Phenotyping Egyptian rootstocks for salinity resistance. WP 2 - Task 2. 1 Genome-wide genotyping and genetic characterization of germplasm resources Partner Biotic stress Abiotic stress UMIL Genotyping Mgaloblishvili progeny (116 genotypes) for resistance against downy mildew. Genotyping M 1 x V. berlandieri progeny (164 genotypes) for drought resistance. ARC Genotyping Egyptian rootstocks for salinity resistance. Kick-off meeting – Meknes, April 1 st-2 nd 2019

Programmed activities: first year WP 3 - Task 3. 1 Development of pre-breeding materials to pyramid resilience traits Partner Biotic stress UMIL Crosses to pyramid downy and powdery mildew Crosses between non-vinifera and vinifera genotypes. resistant traits Crosses between drought and salinity resistant genotypes. ARC Abiotic stress WP 3 - Task 3. 2 Setting up collections in multiple environments Partner UMIL Biotic stress Abiotic stress To be defined…for sure not to be accomplished during the first year. ARC Kick-off meeting – Meknes, April 1 st-2 nd 2019

Deliverables and milestones - D 2. 1: High-quality and high-throughput genotyping data for grape (M 18). - D 2. 2: Identification of genomic regions controlling phenotypic variation for resistance to biotic and abiotic stresses (M 24; M 36). - M 2. 1: High quality DNA samples for WP 2 genetic materials ready for genotyping (M 12). Kick-off meeting – Meknes, April 1 st-2 nd 2019

Thanks for your attention Gabriella De Lorenzis gabriella. delorenzis@unimi. it Rania Ahmed raaniaamed@yooh. it Kick-off meeting – Meknes, April 1 st-2 nd 2019