Fraser Fraser FBUVMModified University of Vermont brothUVM 1LEB

检测程序 ---增菌 目前常用的单核增生李斯特氏菌检测 增菌肉汤主要有半量Fraser肉汤、 Fraser肉 汤（FB）、UVM（Modified University of Vermont broth，也叫UVM 1）、LEB（ Listeria enrichment broth）和BLEB（ buffered Listeria enrichment broth）

FDA：李斯特氏菌属的快速筛选试剂盒 Table 2. Listeria Genus Detection Test Kits Prescribed for Regulatory Screening Ø AOAC Official Method 993. 09. 2000. Listeria in dairy products, seafoods, and meats. Colorimetric deoxyribonucleic acid hybridization method ( GENE-TRAK Listeria Assay). (3, 14) Ø AOAC Official Method 994. 03. 2000. Listeria monocytogenes in dairy products, seafoods, and meats. Colorimetric monoclonal enzyme-linked immunosorbent assay method (Listeria Tek). (5, 15, 32) Ø AOAC Official Method 995. 22. 2000. Listeria in foods. Colorimetric polyclonal enzyme immunoassay screening method (TECRA Listeria Visual Immunoassay [TLVIA]). (6, 30) Ø AOAC Official Method 996. 14. 2000. Assurance (Polyclonal Enzyme Immunoassay Method). (7, 21) Ø AOAC Official Method 997. 03. 2000. Visual Immunoprecipitate Assay (VIP). (8, 22) Ø AOAC Official Method 999. 06. 2000. Enzyme Linked Immunofluorescent Assay (ELFA) VIDAS LIS Assay Screening Method. (9, 23)

FDA：生化鉴定的替代方法 • Vitek Automicrobic Gram Positive and Gram Negative Identification cards (bio. Merieux, Hazelwood, MO) • API Listeria (bio. Merieux, Marcy-l'Etoile, France) which does not require an additional CAMP test • The MICRO-IDTM kit (bio. Merieux, Hazelwood, MO; 1, 24) permits speciation of Listeria isolates if their CAMP reactions are known • The Phenotype Micro. Array for Listeria (Bi. OLOG, Hayward, CA) is another recently introduced kit.

FDA：鉴定单核增生李斯特氏菌的试剂盒 Table 3. Test Kits Useful in Confirming Listeria Isolates as Listeria monocytogenes or not Ø Accu. Probe. TM Listeria monocytogenes culture confirmation test (Gen. Probe, Inc, San Diego, CA) Ø Gene. Trak Listeria monocytogenes test kit (Neogen, Lansing, MI) Ø Probelia Listeria monocytogenes test kit (Bio. Control, Seattle, WA) Ø VIDAS Listeria monocytogenes test kit (bio. Merieux) Ø Transia Plate Listeria monocytogenes (Diffchamb SA, Lyon, France) Ø FDA, SRL application of Niederhauser et al. method for PCR detection and identification of L. monocytogenes Ø BAX Listeria monocytogenes test. (Qualicon, Inc. , Wilmington, DE)

VITEK

API Listeria

BAX

TECRA® UNIQUE PLUS ™ TECRA® UNIQUETM