Fodor Jzsef National Center for Public Health NATIONAL
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION The survey of genetically modified foods on the Hungarian market, with method development and adaptation* Official Control of the Genetically Modified Foods in Hungary * There were only a few similar surveys performed in the EU.
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION The INSTITUTE … Director: Dr. Imre Rodler
Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION Department of Food Additives and Chemical Contaminants Department of Food Chemical Analysis Department of Nutrition Hygiene Department of Food Microbiology Division of Food Additives and Packaging Additives Division of Protein and Vitamin Division of Nutrition Physiology Division of Bacteriology Division of Chemical Contaminants Division of Metal Analysis and Lipid Division of Nutrition Biochemistry Division of Microbiological Biochemistry Veterinary Drug Residues Group Division of Food Product Registration Division of Public Nutrition and Therapeatic Nutr. Entomology Laboratory Cosmetic Group Mycotoxicology Laboratory of Genetically Modified Organisms
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION n Tasks: Ø Ø Ø Ø participates in the preparation of legistlation, with especial regard to the determination of maximum residue limits for foods; regularly supervises the food microbiological and chemical limits and makes proposal for their amendment; performs the comprehensive microbiological, GMO, chemical, toxicological and parasitological laboratory analysis of foods. performs licencing activity with regard to foods meeting particular nutritional demands, nutriments, new food additives, new food packaging materials and cosmetics, and authorizes the use of cleaning agents and disinfectants in food industry; issues certficates attesting that imported foods to be put on the market conform to the public health requirements; issues expert opinion for the manufacture of so-called new foods and foods treated by irradiation, and for the licencing of veterinary medicinal products and pesticeds; provides technical guidance and consulting to assist the work of the NPH&MOS in the field; performs nutrition-physiological and nutritional health studies and conducts consumer awarness campaigns.
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION n GMO related performance: Ø Ø Ø In 1998 the XXVII. Act on gene technology came into force in Hungary, and one year later the Regulation of the Minister of Agriculture (1/1999. (I. 14. )) was published on the execution of the Act, which regulates also the labelling of GM foods. The Hungarian regulation on GMO is conform with that of the European Union. In accordance of the Regulation 1/1999. (I. 14. ) a laboratory for the official control of GM foods was established in the National Institute of Food Hygiene and Nutrition. GMO screening and quantification is carried out in our GMO laboratory in the case of meat products and foods from plant origin.
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION §Dedicated to the official GMO control in the case of meat industry products and products of plant origin (Annex 5 of the FVM regulation No 1/1999. (I. 14. )) Fodor József National Centre for Public Health - National Institute of Food Hygiene and Nutrition GMO Laboratory Address: Postal address: 1097 Budapest, Gyáli út 3/A 1476 Budapest 100, P. O. Box 52
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION The GMO Laboratory …
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION n Instuments: Gene. Amp® 5700 Primus 96 plus quantification detection
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION § GMO team: Boldizsár Vajda Head of GMO Lab Zsuzsanna Bátki Bioengineer Szilvia Baranyi Assistant
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION n Steps of PCR based detection: 1. DNA isolation 2. GMO screening – conventional PCR method, followed by Ø agarose-gelelectrophoresis and Ø restriction digestion of the GMO-positive amplicon 3. GMO quantification – real-time PCR method
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION n Primers: Ø Ø Ø Control primers: q Invertase and zein gene specific primer (in the case of MAIZE) q Lectin gene specific primer (in the case of SOY) q Chloroplast specific primer (for plants) GMO specific primers: q 35 S promoter q NOS terminator Specific primers q MON 810 specific primer (for Yield. Gard maize) q RR specific primer (for Roundup. Ready soybean) q PG gene specific primer (for Flavr. Savr tomato) q Hpt gene specific primer (for GM potato)
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION The PROJECT* … In the framework of Technical Research and Development projects „BIOTECHNOLOGY 2002” *Funded by the Ministry of Education Research and Development
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION n Aim of the project: Investigate the presence of genetically modified components in foods on the Hungarian market Ø Determine the quantity of genetically modified components in foods on the Hungarian market Ø
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION Samples: n Foods containing Ø Soy (100 samples) q. Maize (100 samples) q. Soy and Maize (50 samples) q. Tomato (50 samples) q. Potato (50 samples) q Type of samples Ø Raw materials q. Flour Bakery q. Snacks q. Dry foods Powders q. Meat products q. Sauces q. Drinks q. Other q
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION n GMO screen and quantification: Maize products Soy products Tomato products Potato products
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION n Analysis of the samples: Screening for: q 35 S promoter q. NOS terminator Ø Determination of the GM trait: q. In the case of GMO positive samples Ø Quantification: q. In the case of GMO positive soy or maize Ø
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION n Expected results: Information about the presence and quantity of GMOs on the market Ø Basis of information of consumers Ø Background of the activities of authorities Ø Reliable laboratory background of GMO control Ø Accredited GMO laboratory for the official control of GM foods Ø
„Fodor József” National Center for Public Health NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION - THANK YOU Contact person: Dr. Boldizsár P. Vajda Head of GMO Laboratory E-mail: vaj 4616@ella. hu
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