Flow Cell Injector Tip Fluorescence signals Focused laser

Flow Cell Injector Tip Fluorescence signals Focused laser beam Sheath fluid

Forward Angle Light Scatter Laser FALS Sensor

90 Degree Light Scatter Laser FALS Sensor 90 LS Sensor

Fluorescence Detectors Laser FALS Sensor Fluorescence detector (PMT 3, PMT 4 etc. )

Fluorescence Activated Cell Sorting FALS Sensor 488 nm laser Fluorescence detector Charged Plates Single cells sorted into test tubes - +

Optical Filters Dichroic Filter/Mirror at 45 deg Light Source Transmitted Light Reflected light

Standard Band Pass Filters 630 nm Band. Pass Filter White Light Source Transmitted Light 620 -640 nm Light

Standard Long Pass Filters Light Source 520 nm Long Pass Filter Transmitted Light >520 nm Light Standard Short Pass Filters Light Source 575 nm Short Pass Filter Transmitted Light <575 nm Light

Flow Cytometry Optics PMT 4 Flow cell PMT Dichroic Filters 3 PMT 2 Bandpass Filters PMT 1 Laser

Common Laser Lines 350 300 nm 457 488 514 400 nm 500 nm 610 632 600 nm 700 nm PE-TR Conj. Texas Red PI Ethidium PE FITC cis-Parinaric acid

Propidium Iodide Emisson Excitation 300 nm 400 nm 500 nm 600 nm DNA 700 nm PI

Fluorescein (FITC) Excitation Emisson 300 nm 400 nm Wavelength 500 nm Protein 600 nm 700 nm

Phycoerytherin (PE) Excitation Emisson 300 nm 400 nm 500 nm Protein 600 nm 700 nm

Allophycocyanin (APC) Protein 300 nm 400 nm 500 nm 632. 5 nm (He. Ne) 600 nm 700 nm Excitation Emisson

Typical Research Cytometer (Coulter 753) (1980 s) $200 -300, 000 Detectors Lasers Fluidics Computers Laser Power Supply

Typical Clinical Cytometer Computer System Detector & Mechanical $90 -120, 000 Fluidics

The Conventional Microscope Mechanical tube length = 160 mm Focal length of objective = 45 mm Object to Image Distance = 195 mm