Fig 2 Root sections of Coffea canephora clonal

Fig. 2. Root sections of Coffea canephora clonal variety clone 14 (resistant) infested with Meloidogyne paranaensis. A, C, D, E, and G, Toluidine blue staining; B, F, and H, UV fluorescence observation. A and B, Second-stage juvenile (J 2) nematode located in the root cortex and surrounded by bluestained degraded cells (A) which display a yellow fluorescence under UV illumination (B); C, D, E, F, G, and H, nematodes present in the vascular cylinder. Giant cells can be observed 8 days after inoculation associated with the presence of nematodes (C). A blue-gray fluorescence is seen in cells near the nematode and in walls of vessels (F and H); D, E, and G, parenchyma cells close to J 2 s exhibiting a dark-stained material, suggesting severe alteration of the cytoplasm; E and G, giant cells showing retracted cytoplasm containing numerous vacuoles. N = nematode, HR = hypersensitive reaction indicating cell death (cd), co = cortex, gc = giant cells, vc = vascular cylinder, va = vacuole. Sections visualized under UV light (B, F, and H) and after staining with toluidine blue (A, C, D, E, and G). Published in: Edriana A. Lima; Cleber Furlanetto; Michel Nicole; Ana C. M. M. Gomes; Maria R. A. Almeida; Aldemiro Jorge-Júnior; Valdir R. Correa; Sônia Maria Salgado; Maria A. G. Ferrão; Regina M. D. G. Carneiro; Phytopathology® 2015, 105, 805 -814. Copyright © 2015 The American Phytopathological Society • DOI: 10. 1094/PHYTO-08 -14 -0232 -R