Fertilization Spermatozoa maturation steps The maturation and activation

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Fertilization

Fertilization

Spermatozoa maturation steps

Spermatozoa maturation steps

The maturation and activation of the spermatozoa occur in the following four steps: •

The maturation and activation of the spermatozoa occur in the following four steps: • • Storage in the epididymis Maturation Ejaculation Activation Ascension to the ovary Capacitation Near the oocyte Acrosome reaction

Composition of the seminal plasma Amount 2 -100 ml p. H value 7 -8

Composition of the seminal plasma Amount 2 -100 ml p. H value 7 -8 (light alkaline buffer) Seminal gland secretion 75% of the volume, alkaline fructose-rich secretion (1. 5 -6. 5 mg/ml fructose), phosphorylcholine, ascorbic acid Prostate secretion 20%-25% of the volume, biogenic amine (spermidine, spermine), citric acid, cholesterol, phospholipids, protease to fluidize the ejaculate (fibrinolysin, fibrinogenase) Further components Phosphate and bicarbonate as buffer, prostaglandin,

Human

Human

COW

COW

MARE SOW

MARE SOW

Maturation of the oocyte in the dominant follicle shortly before ovulation

Maturation of the oocyte in the dominant follicle shortly before ovulation

Termination of the first meiosis

Termination of the first meiosis

The secondary oocyte

The secondary oocyte

The follicle that is about to rupture

The follicle that is about to rupture

 Secondary oocyte in metaphase 2

Secondary oocyte in metaphase 2

The acquisition of the oocyte by the fallopian tube

The acquisition of the oocyte by the fallopian tube

Consequences of multiple ovulation • Twin pregnancy • from one mating male • Superfecundation:

Consequences of multiple ovulation • Twin pregnancy • from one mating male • Superfecundation: from two males, after synchrone ovulation • Superfoetation: from two males, after asynchrone ovulation

Formation of a bilaminar germ disk • In the bilaminar primordium of the embryo

Formation of a bilaminar germ disk • In the bilaminar primordium of the embryo (hypoblast or primary endoderm and epiblast) one recognizes in the epithelium of the epiblast a fluid-filled space, the first primordium of the amniotic cavity. • Ventrally, the roof of the still incompletely uncovered primary umbilical vesicle (previously the blastocyst cavity) is formed by the hypoblast.

Develomental differences in early embriogenesis • A: Folding (Carnivores, Rodents, Ungulate (hoofed animal) •

Develomental differences in early embriogenesis • A: Folding (Carnivores, Rodents, Ungulate (hoofed animal) • B: Exocoel formation (Primates, Human, Insectivores)

1. Extra-embryonic mesoblast 2. Extra-embryonic reticulum 3. Primary umbilical vesicle 4. Cytotrophoblast 5. Lacunae

1. Extra-embryonic mesoblast 2. Extra-embryonic reticulum 3. Primary umbilical vesicle 4. Cytotrophoblast 5. Lacunae in the reticulum 6. Hypoblast 7. Heuser´s membrane between hypoblast and mesoblast cells

Formation of the ‘Trilaminar germ disc’ AKA epithelio-mesenchymal transition • Immigrated cells form a

Formation of the ‘Trilaminar germ disc’ AKA epithelio-mesenchymal transition • Immigrated cells form a third germinal layer: the intraembryonic mesoblast • The mesoblast cells wander in all directions: laterally, cranially and caudally. • This middle germinal layer lies between the definitive endoblast and epiblast. • Exceptions are the cloacal membrane as well as the pharyngeal membrane, where the ectoderm and endoderm lie directly opposite each other!