Feedback Control for the Programmable Cell Culture Chip

Feedback Control for the Programmable Cell Culture Chip “Pro. Cell” Felician Ștefan Blaga Supervisor: Paul Pop (DTU Informatics) Co-supervisors: Wajid Minhass (DTU Informatics) and Martin Dufva (DTU Nanotech)

Outline § Early design phases § Design decisions and uncertainties § Modeling uncertainties § Worst-case execution time § Functionality requirements § Problem formulation: mapping optimization § Motivational examples § Multi-objective optimization strategy § Robustness vs. flexibility § Genetic Algorithm approach § Experimental results § Conclusions and message 2

Motivation for Biochips Test tubes Automation Integration Miniaturization Robotics Automation Integration Miniaturization Microfluidics Automation Integration Miniaturization 3

Two types of microfluidic biochips § Continuous-flow biochips: Permanently-etched microchannels, micropumps and microvalves, electrokinetics, etc. § Digital microfluidic biochips: Manipulation of liquids as discrete droplets Multiplexing Mixing: Static, Diffusion Limited Biosensors: Optical: SPR, Fluorescence etc. Electrochemical: Amperometric, Potentiometric etc. 4 4

Application area: cell culturing § TODO Some text about cell culturing 5

Pro. Cell setup § Requirements: § 1 § 2 § Components § 1 § 2 6

Pro. Cell control diagram Explanations, color change… PC Microscope Motors controlling the pumps Signal adaptor Lab. Jack Procell Prototype Explanations 7

Pro. Cell prototype The biochip is fabricated using micro-milling prototyping 8

Zeiss Microscope § Something about the microscope and the Axio. Vision Software § Something about fluorescence 9

Control box § Something about the control box, and difficulties in putting everything together (you had to order the box, etc. ) § What you will cover next: § Lab. Jack § Signal adapter board 10

Lab. Jack § What is a Lab. Jack? (copy paste from thesis) § How is it controlled? 11

Signal adapter board § Some explanations about the motors § Use sub-bullets if needed § Something about the board and why you needed the counter Ripple counter Step motor Faulhaber AM 1524 12

Three types of control 1. Direct control § Short text about it—take it from thesis and be succinct 2. Scenario-based control § Short text about it 3. Feedback control § Short text about it 13

Scenario-based control § Focus on VBA § Tell us what is on the left § Tell us what you did with VBA 14

Feedback control Main part Lab. Jack control Scripts control § Explain the feedback control § Tell us about VBA Excel interface § Maybe we need two slides? Ref R P M 15

Evaluation: direct control § What do we see in the picture § How is the control achieved 16

Evaluation: scenario-based control § What do we see in the picture § How is the control achieved 17

Evaluation: feedback control § No Feedback Imeasured (a. u. ) No feedback 1800, 00 1700, 00 1600, 00 1500, 00 1400, 00 1300, 00 1200, 00 1100, 00 1000, 00 900, 00 800, 00 700, 00 § What do we see in the picture § How is the control done R 2 = 0, 9875 0 10 20 30 40 Pump percentage( %) 50 60 Imeasured (a. u. ) Feedback control 1600, 00 1500, 00 1400, 00 1300, 00 1200, 00 1100, 00 1000, 00 900, 00 800, 00 700, 00 600, 00 500, 00 R 2 = 0, 9995 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 Iset point(a. u. ) § With feedback § What do we see in the picture § How is the control done § What is the conclusion 18

Evaluation: feedback control § Remind what is fluorescence § What do we see in the picture Fluorescence § How was the control done 2000 1500 1000 § Conclusion: why is it good 500 0 0, 5 4 7, 5 11 14, 5 18 21, 5 25 28, 5 32 35, 5 39 42, 5 46 49, 5 53 56, 5 60 63, 5 67 70, 5 74 77, 5 81 84, 5 88 91, 5 Imeasured (a. u. ) 2500 Time (min) 19

Conclusions § Pro. Cell setup: cell culturing biochip, microscope, proposed control box § Several control approaches, using VBA and the control box § Direct control § Scenario-based control § Feedback control § The control solution has been systematically evaluated and tested § The solution is successfully used in the field § § DTU Nanotech Danish Cancer Society University of Oslo Bioneer § The work done in thesis has been partly reported in two scientific papers 20