Family Neisseriaceae By Prof Dr Moussa Commensally present
Family Neisseriaceae By: Prof. Dr. Moussa
Commensally present in: Neisseria �Conjunctiva �Throat �Mucous membrane �Rectum, cervix and uterus. are
Pathogenic Neisseria are: �Neissseria meningitides. �N. gonorrhea.
Pathogenic Commensal Nutrient agar Don’t grow Grow At 22°C Don’t grow Grow CO 2 need Need Don’t need Biochemical Reaction Glucose Maltose Lactose Sucrose +ve G +ve / M –ve -ve All are +ve or All are –ve Gonorrhea meningitis None Pathogenecity
Pathogenic Neisseria Morphology: �Gram –ve �Diplococcic – kidney shape �Non-motile �Non-sporulated �Capsulated Habitat: �N. meningitis → carried in nasal pharynx �N. gonorrhea→ no carrier, if present considered patient
Culture �Can’t grow in N. agar or Macconkey �They are fastidious microorganism (Need blood to grow) best chocolate agar. �The selective media for isolation of pathogenic Neisseria →Thayer Martin Medium → (chocolate agar + antibiotic VCN) vancomycin Colistin Nystatin �Grow at 37°C after 24 hr aerobically. �Non hemolytic �Need CO 2 (5 -10%)
What is gonorrhea? It is venereal disease (sexually transmitted) � Acute : male → severe urethral discharge. Female → Cervical discharge. � Chronic : Male →morning drops of pus and orchitis lead to sterility & systemic infection. Female →inflammation of fallopian tubes (salpingitis) Inflammation of ovary (oophoritis). Cervical discharge. Antibiotic Colistin resistance
N. gonorrhea N. meningitidis Disease Venereal disease (gonorrhea) Not venereal disease (Meningococcal meningitis) Infection High morbidity and low mortality High mortality and low morbidity Acid production from: Glucose Maltose Lactose sucrose +ve -ve -ve +ve -ve
THANK YOU
- Slides: 9