Fabrication of Biosensors and Lab Testings III Jack

Fabrication of Biosensors and Lab Testings III Jack Nguyen Xiaodong Ma 05/14/2008

For today Theory/Background, Testings – Jack Result, other two experiments– Xiaodong

OBJECTIVES OF PROJECT: Fabrication of FP Biosensors Repeated Analyze antigen/antibody (Influenza A/anti-Influenza A ) interactions Test different Techniques / Antigens MATERIALS: Fiber Optic Strands, CTS Machine, Matlab, PMMA/Acetone solution, Protein G’(10µg/ml), 2% BSA in PBS, Influenza A(20µg/ml), Anti-Influenza A Ig G(20µg/ml), Distilled water, 3 aminopropyltriethoxysilane, Acetone, Ethanol, Microscope, Light, Controllable stage

Budget: $0, all materials provided by UML

Team Roles TASKS RESPONSIBILITY Lab Reports / PPT Jack, Xiaodong Fabrication of Biosensors Jack Lab Testings Jack, Xiaodong Data Analysis Xiaodong

Schedule Followed TASKS Sensors Fabrication Repeat Lab Testings Present. II/Report Present. III/Report Data Analyzation Test Diff. Antigens Analyze Diff. Anti. Finalization All Testings START 4/01/08 4/09/08 4/16/08 4/10/08 4/30/08 5/02/08 5/10/08 END 5/13/08 4/16/08 5/14/08 5/12/08 5/13/08 5/14/08

Optical Fiber with PMMA

Principle of FP cavity sensor.

Schematic diagram showing the fringe shift

Schematic graph of biosensor Influenza A Primary Antibody Pro. G Optical Fiber poly(methyl 2 methylpropenoate)

Fabrication of Biosensor

Procedure SENSOR PMMA PBS BSA WATER Pro. G WATER PBS Ig. G Inf. A PBS WATER PBS DATA

Time Duration Fabrication FP sensor : 30 Minutes PMMA: 2 Hours Pro. G: 1. 5 Hours BSA: 1. 5 Hours Ig. G: 1. 5 Hours Influ. A: 1. 5 Hours Total PBS: 5 Hours Total Hours for a typical lab test: 13 -16 Hr When a lab test fails, we start all over with the FP sensor fabrication

Experimental setup Matlab Program Chiller CTS Biomaterials Fiber

Experimental setup Polymer tube Fiber Glass tube

Fringes in the air

Fringes in the PBS

Result I without BSA

Result II without BSA

Result III with BSA

PMMA Sheet

Experimental setup Stage Detector Stage power Connector to CTS Sensor

Result

Si. O 2 /Si VIS spectrometer

Experimental setup Light Source VIS Spectrometer Detector Si. O 2/Si

Experimental steps • Wash out the dust of Si. O 2/Si substrates several times by DI water. • Wash the Si. O 2/Si substrates by sonication in acetone, ethanol and DI water for 15 min, respetively. • Put the cleaned and dry Si. O 2/Si substrates in 2% solution of 3 aminopropyltriethoxysilane in acetone (3 -aminopropyltriethoxysilane : acetone =1 : 49 by volume ) at room temperature for 30 seconds. • Measure the substrates’ spectra by VIS spectrometer after Si. O 2/Si substrates dried. • Drop Pr. G’ on Si. O 2/Si substrates for 1. 5 h, wash by DI water 3 times, air-dry and detect its spectra. (For comparison experiment, drop BSA on the substrates first and then wash and detect spectra. ) • Drop anti-Inf. A Ig. G on the surface for 1. 5 h, wash by DI water 3 times, air-dry and test its spectra. (For comparison experiment, drop Pr. G’ on the surface with BSA and wash, dry detect spectra. ) • Drop Inf. A on the surface for 1. 5, wash by DI water 3 times, air-dry and then measure its spectra. • Analyze the spectra of two groups experiment.

Results

Results

Results

Results