Fabrication and Characterization of Human Serum Albumin HSA

Fabrication and Characterization of Human Serum Albumin (HSA) Microcapsules Christopher Blais and Nivedita Gupta Department of Chemical Engineering Optimization of HSA Capsule Fabrication Motivation Parameters Studied 10 min 20 min 30 min 60 min Study capsules in constraining geometries • trxn - Reaction time 20% 10 min, 20 min, 30 min • Carbonless Paper Longer reaction times and higher HSA concentrations yielded more robust capsules 30% • Textiles 10% , 20%, 30% • Cosmetics • Pharmaceuticals Q • CHSA - HSA concentration (percent by weight) 10% • Food Relevant Parameters • Living cells Dilatation Studies 3% Na. Cl 2. 5 Volume (mm 3) 2. 0 Fabrication of HSA Capsule 10% 5. 5% 3% 0. 5 0 500 Time (min) 1000 1500 Smaller volume change of capsules observed at lower saline solution concentrations Aspiration Studies Crosslinking Reaction in Terephthaloyl Chloride forms membrane Store capsules in Isotonic Saline Solution HSA capsule strength affected by reaction time and solution concentration 0. 004 Ca = 0. 42, k = 1. 32 Ca = 0. 83, k = 1. 30 Numerical Simulation of a Capsule 3 m. L/min, 25% HSA Capsule in 5. 5 w/v salt solution Experimental setup 6. 4 k. Pa 0. 003 t = 0 s 13. 9 k. Pa 0. 002 t = 0. 96 s 0. 001 0 t = 2. 60 s 0 0. 005 0. 015 0. 02 Area Dilation (DA/A 0) 0. 025 25% HSA capsules show a compressibility modulus (K) of 164. 5 m. N/m Drop Deformation Studies: Experimental versus Numerical g = 0. 28, c = 1, l = 0. 02, Re/Ca= 0. 53 dd 9. 86 k. Pa 0. 005 Isotropic tension (N/m) 10 c. St Silicone oil Drain Bulk and wash with IPA and DI Water 0. 006 NH 2 HSA Drop formation in TJunction z=0 Future Work: Capsule Deformation Studies 1. 5 1. 0 20% wt. HSA in DI water 10% Na. Cl Characterization of HSA Capsule Flow induced deformation of diseased cells can be used to diagnose diseases g = 0. 28, c = 1 , l = 0. 02, Re/Ca = 0. 53 0. 03 Capsules are too rigid to pass through current geometry without lysing. Larger channel geometry and lower required Summary • HSA microcapsules successfully fabricated • Robust capsules for 25% HSA solution with 30 minute reaction time • Dilatation and aspiration experiments set up to characterize capsules Further Research • Conduct deformation studies to determine critical Elasticity number at which capsule breaks • Analyze how it changes with constraining geometry Acknowledgements Numerical simulations predict breakup at lower Ca Interfacial tension may be underestimated UNH Hamel Center for Undergraduate Research Robert Carroll (Ph. D 2014 UNH) Brian Zukas (Ph. D 2016 UNH)