EXPERIMENTAL PLAN 1 root germination root tip size

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EXPERIMENTAL PLAN 1 - root germination: root tip size 1 -1. 5 cm 2

EXPERIMENTAL PLAN 1 - root germination: root tip size 1 -1. 5 cm 2 - fixation : killing all cell organelles except nucleus CORNOY SOLUTION 3 : 1 absolute alcohol: glacial acetic acid add the root to cornoy solution for 24 hrs then adding 70% ethyl alcohol and storing in the refrigerator

3 - hydrolysis : dissolution cells from each other by adding 1 N HCL

3 - hydrolysis : dissolution cells from each other by adding 1 N HCL at 60°C for 6 -8 min 4 - staining: leuco basic fuchsin is used to stain the roots. Store over night in brown bottle then add animal charcoal(1 g) and filter ; take the filter and store

5 - mounting : * Preparing Temporary slide: cut root tip Add drop of

5 - mounting : * Preparing Temporary slide: cut root tip Add drop of 45% acetic acid for clearing Put cover Spread and examine * Preparing permanent slide: by egg albumin on the cover After mounting put slide in 40% ethyl alchohol Take the cover and add 95% ethyl alcohol for 3 minutes and 100% alcohol for 3 min Add euporal on the cover Dry in the oven at 40 digress

https: //www. youtube. com/watch? v=LQF_ih. RA _p. A

https: //www. youtube. com/watch? v=LQF_ih. RA _p. A

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