EXPERIENCE WITH THE USE OF Acidosalus A FERMENTED

EXPERIENCE WITH THE USE OF Acidosalus® - A FERMENTED LACTIC PROBIOTIC WITH Lactobacillus acidophilus M. Brlečić1, I. Kosalec 2, N. Vidić1, S. Večerina-Volić3, V. Škerk 4 1 "Nature cures", Vlaška 40, Zagreb 2, Pharmaceutical-Biochemical Faculty, Schrottova 39, Zagreb 3 Phoniatrics centre Šalata at the ORL Clinic Šalata, Šalata 2, Zagreb 4 Clinic for infectious diseases ‘Dr. Fran Mihaljević’, Mirogojska 8, Zagreb

Lactobacillus and balance of microbes ally c i n i l re c e w s t effec ne and s t i ici and d k e l i m ić v al sm o n u r r l i a e h t ić K , in idop y m c g o a o T ch l , r o a 0 a r n 6 e u u 9 s , r t re r. K as 1 s of ORL a D b h y. e t l f r r f o g a o r • As e at section itals in Za liged to P tribution p n b d teste ics in hos we are o ientific co h c tr pedia is researc ces and s roject p th en • For ve experi on for our ti ti • Posi ed motiva d provi Acidosalus®

Lactobacillus and balance of microbes ¡ ¡ ¡ Lactobacillus is a constituent part of the commesal flora of the dynamic gastrointestinal system and the vagina Colonization immediately after birth (through milk) Balance essence of homeostasis Fedorak et al. , Curr Opin Gastro 20(2004) Acidosalus®

Bacteria Lactobacillus sp. ¡ ¡ Gram-positive bacteria of the Lactobacillaceae family, which includes around 120 species Commesal flora, but used also as a probiotic Lactobacillus lactis, L. plantarum, L. rhamnosus, L. bulgaricus, L. helveticus L. acidophilus complex dominant bacteria (L. acidophilus, L. crispatus) Acidosalus®

Probiotics are: Live microorganisms which, when used in the sufficient amount, have positive healing effect on the host ¡ Probiotics change the composition and metabolic activity of the microflora or affect the immune system, which has positive effect on the host’s health ¡ Acidosalus® FAO/WHO: www. fao. org/es/ESN/Probio/probio. htm Macfarlane et al. , Curr Opin Infect Dis, 15 (2002).

Acidosalus® ¡ Protective in situ effect of Lactobacillus is described through a series of mechanisms: Competition with pathogenic bacteria for receptors ¡ Coaggregation impossible adherency of pathogens ¡ Creation of antimicrobial substances: lactic and acetic acid ( p. H), H 2 O 2, diacetyl and bacteriocin: lactacyd, acidophilucin, acidophilin ¡ Boris et al. , Infect Immun 66 (1998)

Favourable therapeutic effect of Lactobacillus: Therapeutic effect presupposes peroral use of live or lyophilized bacteria aiming at colonization of the gastrointestinal system (oral cavity, duodenum, small and large intestine) or throat and oral cavity ¡ Intravaginal instillation –local application of live or lyophilized bacteria aiming at colonization of the vaginal epithelium ¡ Acidosalus®

Studies on beneficial effects of Lactobacillus treatment ¡ Vulvovaginitis caused by candidas or bacteria Van Kesell et al. , Obstet Gynecol Survey, 2003 Jeavons JOGNN, 2003 Acidosalus®

Studies on beneficial effects of Lactobacillus treatment ¡ Infections of the gastrointestinal system : Helicobacter pylori (ulcer) Clostridium difficile (pseudomembranuous colitis) Enteropathogenic bacteria (diarrhea) Candida albicans Rotaviruses Infestation with the parasite Giardia lamblia ¡ Changes in microbial flora after antibiotic treatment, diarrhea and non-infectious diseases (Morbus Crohn, and oth. ) Madden et al. , Inter Immunopharmacol 2005

Acidosalus® Lactic preparation with minimally 1011/m. L live Lactobacillus acidophilus

Experiences with use of Acidosalus®: ¡ 80 patients with infectious and non-infectious diseases: urogenital (60%) gastrointestinal (20%): upper respiratory sytem and oral cavity (20%)

Acidosalus® Application of Acidosalus® : ¡ Depending on the type and localization of the disease: l l Peroral (regime): Vaginal instillation

Application of Acidosalus® : Why are laryngology and phoniatrics chosen? • Interconnections of pathology of the gastrointestinal vs. respiratory system • GERD and GLRD as obvious examples of effects of the reflux mechanism from the gastrointestinal to the respiratory (phonatory) system • Occurence of certain pathological conditions which cannot be eradicated neither by conventional nor surgical therapy GERD and GLRD Papillomatosis (HPV) of the larynx, hypopharinx, oral cavity Fungal infections in these areas Possibility of objective digital documentation

Monitoring of patients in laryngology and phoniatrics: 1. Subjective changes 2. Objective: digital endoscopic images and video clips

Monitoring of patients: Subjective changes (a questionnaire, but non-specific) ¡ Objective changes ¡ GERD (gastroesophageal reflux disease) GLRD (gastrolaryngeal reflux disease) GLRD or GLRD and HD

Possible consequences of GERD or GLRD

Back granulomas

OBJECTIVE TESTS ( digital endoscopic images) Various degrees of back laryngitis

OBJECTIVE TESTS digital endoscopic images before and after therapy(8 out of 10 patients) Before ARth and AS After ARth and AS Only AR th. P r i After AS Before AS Only AR th

OBJECTIVE TESTS Acidosalus only Before Acidosalus After Acidosalus

OBJECTIVE TESTS Colonization of fungi at the base of the tongue Digital endoscopic image before AS (resistance to antimycotic) Digital endoscopic image after AS

OBJECTIVE TESTS Human papillomavirus of the larynx and hypopharinx Before th with CO 2 Laser BEFORE AC AFTER AC After th with CO 2 Laser and AS

Widening of use ¡ Incorporation of Acidosalus® into bases: ¡ Application in dermatology

Widening of research and use Further research • Virosalus R in the base of goat milk with addition of garlic • Pekisalus in the base of fresh beetroot juice with addition of garlic

Recommendations Conclusions ¡ Based on the testings of 80 patients, it can be concluded that use of the lactic probiotic preparation Acidosalus® with bacteria Lactobacillus acidophilus significantly improved the patients’ health condition at the level of subjective positive changes and with no side effects

CONCLUSIONS • Using techniques of objective test evaluation for parts of the respiratory, gastrointestinal and phonatory systems, it is possible with great accuracy to monitor effects after therapeutic treatment • These are techniques of digital visualization in both static and dynamic form, including slow motion, and these are executed at the Phoniatrics Centre of the ORL CLINIC Šalata, the Reference Centre of the Croatian Health Ministry • The authors of this research gave such methodology priority over the questionnaire in this review Acidosalus®

CONCLUSIONS Although the number of subjects should be increased, the presented objective clinical documentation shows the effects of Acidosalus® in cases of complex clinical conditions which result from disturbed local or overall immunity of the organism, i. e. biological balance. PROBIOTICS ARE PRECISELY THAT WHICH RESTORES THE BALANCE Acidosalus®