EVPP 110 Molecules Processes of Life Activities Microscopy

EVPP 110 Molecules & Processes of Life Activities: • Microscopy • Water Properties / Alteration by Pollutants Week of February 5 th, 2018

Group photo © Xiatong Liu “Frozen Raindrops” © Tina Le

Reminders �Each person must submit their own Weekly Data Sheets and Weekly Write-Ups. �Even though the WDSs are done as a group, I cannot assign you a grade unless you upload the file to Black. Board from your own account. �Please include your full name, my name and the section number on ALL pages submitted to Black. Board. �Black. Board does not organize the submissions by instructor and I want to make sure I see your assignment.

Molecules & Process of Life Activity 1 – Microscopy

Eyepiece Compound Microscope Neck Nosepiece Objectives Stage controls Macrofocus Stage �Tutorial: �http: //www. udel. edu /biology/ketcham/mi croscope/index. html Diaphragm Light source Base Microfocus Light adjustment

�Example of type of image seen with compound microscope.

How to prepare a wet mount: Cover slip Lower slowly

�Observe prepared letter “e” slide (Fig 1. 1). �Prepare stained yeast culture slide (Fig 1. 2). Unaided eye Compound microscope Figure 1. 1. Sketch of prepared letter “e” slide as viewed with unaided eye and compound microscope. Unaided eye Compound microscope Figure 1. 2. Sketch of yeast culture, stained with crystal violet, as viewed with the unaided eye and compound microscope.

�Prepare algae culture slide (Fig 1. 3). �Prepare wet mount of underside of plant leaf (Fig 1. 4). Unaided eye Compound microscope Figure 1. 3. Sketch of algae culture, unstained, viewed with unaided eye and compound microscope. Unaided eye Compound microscope Figure 1. 4. Sketch of thin section of plant leaf underside as viewed with unaided eye and compound microscope.

Eyepiece Dissecting microscope Zoom Objective Focus Upper light Lower light

�Example of type of image seen with dissecting microscope.

�Observe whole plant leaf (Fig 1. 5). �Observe whole insect (Fig. 1. 6). Unaided eye Dissecting microscope Figure 1. 5. Sketch of whole plant leaf viewed with unaided eye and dissecting microscope. Unaided eye Dissecting microscope Figure 1. 6. Sketch of invertebrate viewed with unaided eye and dissecting microscope.

�Observe whole rock (Fig. 1. 7). �Observe whole bracket fungus (Fig. 1. 8). Unaided eye Dissecting microscope Figure 1. 7. Sketch of whole rock viewed with unaided eye and dissecting microscope. Unaided eye Dissecting microscope Figure 1. 8. Sketch of whole bracket fungus viewed with unaided eye and dissecting microscope.

Molecules & Process of Life Activity 2 – Properties of Water and Alteration by Pollutants

�Fill 140 m. L plastic container with tap water (unpolluted) until bulging. �Predict number of 1. 25 m. L spoons of powdered sugar that can be added to the water before it spills. �Add sugar one spoon at a time until water spills. �Repeat procedure with simulated polluted water. Table 2. 1 Part A. # of 1. 25 m. L spoons of powdered sugar added to 140 m. L container of water before water spills: Unpolluted Water Predicted Actual Explanation: Water properties demonstrated: Polluted Water Predicted Actual

�Fill one balloon with tap water. �Fill 2 nd balloon with air (from pump) until same size as 1 st balloon. �Predict number seconds it will take for air balloon to pop (Table 2. 2). �Hold air balloon over candle, record number of seconds until it pops. �Repeat procedure (including prediction) with water balloon. Table 2. 2 Part B. Prediction Actual # seconds until air balloon pops after contact with flame: Explanation: # seconds until water balloon pops after contact with flame: Explanation: Water properties demonstrated:

�Predict and record number drops of unpolluted water that can be placed on a penny before the water spills over (Table 2. 3). �Count actual number of water drops record your results. �Repeat procedure (including prediction) with simulated polluted water. Table 2. 3 Part C. # water drops that can be placed on a penny before the water spills over: Explanation: Unpolluted Water Prediction Actual Polluted Water Prediction Actual Water properties demonstrated:

�Fill weigh boat with tap water until water is level with (but not bulging above) rim. �Gently place sewing pin on water surface. �Observe and record appearance of water surface adjacent to pin (Table 2. 4). �Dip tip of a toothpick in liquid soap (pollutant), touch soapy tip to water surface and observe what happens. �Record observations (Table 2. 4). Part D. Table 2. 4 Prior to Pollutant Appearance of water surface adjacent to sewing pin: Behavior of the sewing pin on/in water: Explanation of behavior: Water properties demonstrated: After Pollutant

What’s Due �Weekly Data Sheets: �P. 47, 51, 55, 59, 69, 73 Power. Point available at: https: //eeltown. org/evpp-110 -spring-2018/ OK to turn in hard copy �Weekly Write-Ups: �P. 63, 77 -78 Post to Black. Board