Evolution of Technology Agitator technology Biopharma Process www

Evolution of Technology Agitator technology

Biopharma Process www. zeta. com page 2 Evolution of Technology

General Setup - Bottom Mounted Agitator BMRT Retaining bolt with O-ring agitator head, with integrated magnets Bearing: female bearing Si. C (black) male bearing Zr. O 2 (white/yellow) O-ring weld plate, with containment shell Drive magnets Shaft intermediate flange (extension, standard scope of supply) worm geared motor (made from stainless steel) www. zeta. com Drive options available, for instance s/s drives Seite 3 Evolution of Technology

Invisible but indispensable - Unique ZETA bearing design - Si. C - rotating bearing featuring lubrication channels to minimize wear - Zr. O 2 - static bearing with high stress tolerance - Anit-twist Protection - preventing rotation of the male bearing - weld plate, containment shell, pivot - showing improved stability being one turned part - Product wetted parts - stainless steel grade 316 L - according to FDA and USP Cl. VI www. zeta. com Seite 4 Evolution of Technology

Hermetically sealed Retaining bolt (O-Ring sealed) Inner bearing (Zr. O 2) Option: removable containment shell („Plug In“) Weld plate with containment shell and bearing pivot www. zeta. com Seite 5 Evolution of Technology

Cleanability Agitator design ensuring cleanability and sterilization § Complete drainability § Open construction with no dead ends according to ASME BP big gaps between rotor, hubs and shaft shoulder Consideration of a certain gap between inner & outer bearing § www. zeta. com Grooves in outer bearing to ensure better lubrication & cleanability performance Page 6 Evolution of Technology

Lowering device Advantage: • No drive removal necessary . . • easy and safe mounting of the impeller head Driving magnet position during operation Lowered driving magnets during maintenance Lowering device permanently mounted www. zeta. com Page 7 Evolution of Technology

Speed Measurement Additional magnet inside Impeller system Speed sensor: magnetic proximity switch (signal processing not included) Standard for agitators in Ex Zone 0/1 (for safety reasons) www. zeta. com Page 8 Evolution of Technology

Agitator Types Mixing capacity in Torque max. ø Impeller Nm mm BMRT 35 0. 3 80 2 100 BMRT 50 0. 5 80 5 BMRT 80 M 0. 8 105 50 140 350 BMRT 125 130 100 500 BMRT 400 4 165 200 1200 BMRT 800 8 190 700 2500 BMRT 1300 13 250 4500 BMRT 2600 26 300 1000 2000 BMRT 5000 50 3500 15000 BMRT 10000 100 400 6000 25000 BMRT 17000 170 450 10000 40000 BMRT 21000 210 500 15000 55000 Model no. www. zeta. com litres (depending on application) min max 8000 page 9 Evolution of Technology

Customer benefits www. zeta. com Seite 10 Evolution of Technology

Selected customer references Year company application QTY agitators Type 2013 Singapur CHO Fermentation 30 diverse 2013 Thailand API Production 36 BMR 2015 Germany Plasma fractioning 20 BMRT 2015 Singapur CHO Fermentation 25 diverse 2015 Germny Plasma fractioning 51 BMRT 2015 South Korea API Production 74 BMRT 2015 Germany CHO Fermentation 31 diverse 2016 China CHO Fermentation 6 BMRF 2016 China Media-/Buffer prep. Harvest, Chroma, UF 31 BMRT 2016 Austria Plasma fractioning 23 BMRT 2017 Austria Cell Culture Fermentation 20 BMRF 2017 Austria Cell Culture Fermentation 24 BMRT www. zeta. com page 11 Evolution of Technology

Thank you for you attention! ZETA Biopharma Gmb. H Zetaplatz 1, 8501 Lieboch/Graz, Austria www. zeta. com Page 12