Evolution of POC Informatics Source Medical Automation Systems

Evolution of POC Informatics Source: Medical Automation Systems (MAS) Αθήνα 7 -9 Οκτωβρίου 2010, 9ο Πανελλήνιο Συνέδριο ΕΕΚΧ - ΚΒ 26

486 Alcohol, Breath 027 Luteinizing Hormone (LH) 021 Triglyceride 392 Allergen Specific Ig. E / 122 Glucose Panel 090 Methadone 188 Uric Acid 041 Amphetamines 116 Glucose Monitoring Devices 92 Methamphetamine /Amphetamine 448 Urinary Protein, Qualitative 103 Barbiturates 072 Glucose, Fluid 100 Methamphetamines 186 Urine Dipstick Or Tablet Analyte 101 Benzodiazepines 422 Glucose, Urine 256 Methylenedioxymethamphetam ine 125 Urine h. CG By Visual Color Tests 409 Bilirubin, Urine 208 Glycated Hemoglobin, Total 003 Microalbumin 264 Urine Qualitative Bilirubin 084 Cannabinoids (THC) 172 Glycosylated Hemoglobin (Hgb A 1 c) 143 Morphine 265 Urine Qualitative Dipstick Blood 171 Chloride 261 h. CG, Serum, Qualitative 087 Opiates 266 Urine Qualitative Glucose 161 Cholesterol 370 h. CG, Urine 030 Ovulation Test (LH) Visual Color 001 Urine Qualitative Ketone 086 Cocaine Metabolites 121 HDL Cholesterol 285 Oxycodone 268 Urine Qualitative Nitrite 159 Creatinine 016 Hemoglobin 408 p. H, Urine 269 Urine Qualitative Dipstick p. H 494 Estrone-3 Glucuronide 460 Hemoglobin A 1 085 Phencyclidine (PCP) 270 Urine Qualitative Protein 274 Fecal Occult Blood 124 Ketone, Blood 245 Protein, Total (Urine) 271 Urine Qual. Urobilinogen 249 Fern Test, Saliva 352 Ketone, Urine 364 Semen 410 Urobilinogen, Urine 026 Follicle Stimulating Hormone 024 Lactic Acid (Lactate) 246 Tricyclic Antidepressants 042 Vaginal p. H Αθήνα 7 -9 Οκτωβρίου 2010, 184 Fructosamine 9ο Πανελλήνιο Συνέδριο ΕΕΚΧ - ΚΒ 29

Αναδυόμενες μέθοδοι POCΤ βασισμένες στη Μοριακή Βιολογία n n Στα μοριακά POCT δύο σημαντικές επιτυχίες υπήρξαν οι ταχείες δοκιμασίες: u MRSA (Multi-drug Persistent Staphylococcus Aureus). u Clostridium Difficile στα κόπρανα με real-time PCR. Πολλά φαίνεται να υπόσχονται οι ισοθερμικές μέθοδοι: u SMAP (SMart Amplification Processes). u SMART (Signal Mediated Amplification of RNA Targets). u LAMP (Loop-mediated isothermal AMPlification). u HDA (Helicase-Dependent Amplification). Αθήνα 7 -9 Οκτωβρίου 2010, 9ο Πανελλήνιο Συνέδριο ΕΕΚΧ - ΚΒ

Μέθοδος ανίχνευσης χωρίς επισήμανση με Whispering - Gallery Mode (WGM) Ι Frank Vollmer, Stephen Arnold, Whispering-gallery-mode biosensing: Label. Free detection down to single molecules, Nature Methods 5, 591 -596 (2008). Αθήνα 7 -9 Οκτωβρίου 2010, 9ο Πανελλήνιο Συνέδριο ΕΕΚΧ - ΚΒ

Μέθοδος ανίχνευσης χωρίς επισήμανση με Whispering - Gallery Mode (ΙΙ) F. Vollmer, S. Arnold, D. Keng, Single virus detection from the reactive shift of a whispering-gallery mode, PNAS, December 30, 2008, vol. 105, no. 52 20701– 20704 Αθήνα 7 -9 Οκτωβρίου 2010, 9ο Πανελλήνιο Συνέδριο ΕΕΚΧ - ΚΒ

Abstract of CN 101709261 (A) 2010 -05 -19 Applicant(s): UNIV HONG KONG SHENZHEN The invention discloses a microfluidic microbead array chip and application thereof in virus analysis, and belongs to the field of biological application of a miniaturized total analysis system. The microfluidic microbead array chip consists of a microbead fixed microstructure array, a microbead transport channel, a reagent transport channel and a microstructure array coverage channel. When highmicrofluidic microbead array chip consists of a microbead fixed microstructure array, a microbead transport channel, a reagent transport channel and a microstructure array coverage channel. When high-sensitive virus analysis is performed, virus molecules in a serum sample is prepared into a biotin doped target RNA sequence through PCR and in vitro transcription process, and flows to a testing area sensitive virus analysis is performed, virus molecules in a serum sample is prepared into a biotin doped target RNA sequence through PCR and in vitro transcription process, and flows to a testing area of the chip to react with a functional microbead array; function modified microspheres can specifically identify and capture target sequences; a quantum dot labeled reagent are introduced; surfaces of the chip to react with a functional microbead array; function modified microspheres can specifically identify and capture target sequences; a quantum dot labeled reagent are introduced; surfaces of micro-particles are combined with quantum dots because of specifically capturing; and detection and quantization are performed by a fluorescence imaging method. The microfluidic microbead array chip micro-particles are combined with quantum dots because of specifically capturing; and detection and quantization are performed by a fluorescence imaging method. The microfluidic microbead array chip provided by the invention has the advantages of simple process, high detecting sensitivity and quick detection, and provides an effective studying and detecting means for high-sensitive virus analysis. Data supplied from the espacenet database — Worldwide A quantum dot is a semiconductor whose excitons are confined in all three spatial dimensions. As a result, they have properties that are between those of bulk semiconductors and those of discrete molecules. An exciton is a bound state of an electron and hole which are attracted to each other by the electrostatic Coulomb force. It is an electrically neutral quasiparticle that exists in insulators, semiconductors and some liquids. The exciton is regarded as an elementary excitation of condensed matter that can transport energy without transporting net electric charge. Αθήνα 7 -9 Οκτωβρίου 2010, 9ο Πανελλήνιο Συνέδριο ΕΕΚΧ - ΚΒ 48

Microfluidic micro-bead array chip and application thereof in virus analysis CN 101709261(A) 19/05/10 Αθήνα 7 -9 Οκτωβρίου 2010, 9ο Πανελλήνιο Συνέδριο ΕΕΚΧ - ΚΒ 49