Evolution in Real Time Bacteria and Antibiotic Resistance

Evolution in Real Time: Bacteria and Antibiotic Resistance Lab Report 30 points

Hypothesis (4 points) Predictions: What bacteria phenotype (ampicillin sensitive E. coli and/or ampicillin resistant E. coli) will grow on each plate (2 LB and 2 LB-Amp) and why? a. This can even be 4 separate statements (1 for each plate) b. Be thorough in your explanation (why you predict what you predict)

Purpose (4 points) State the purpose for each setup a. Day 1 b. Day 2 -7 c. Day 8 d. Day 9

Procedural Error (4 points) State any procedural errors, differences, or inconsistencies that your group encountered at any point in the experiment a. Cite the correct or original step b. Indicate the effect or predicted effect on results

Data table (6 points) a. number of colonies b. types of colonies c. quantities of materials you used

Data Analysis (6 points) 1. Explain phenotype present on each plate using data 2. Compare both LB plates a. Explain differences in the colonies on each plate b. Explain similarities in the colonies on each plate 3. Compare both LB-Amp plates a. Explain differences in the colonies on each plate b. Explain similarities in the colonies on each plate

Conclusion (6 points) 1. Explain if your data supports or rejects your hypothesis. Support your explanation using data. 2. Explain how your results is an example of evolution by natural selection (refer to the data and four factors).