Essential Cell Biology Third Edition Chapter 15 Intracellular

Figure 15 -1 Essential Cell Biology (© Garland Science 2010)

Figure 15 -2 Essential Cell Biology (© Garland Science 2010)

Table 15 -1 Essential Cell Biology (© Garland Science 2010)

Types of intracellular transport Figure 15 -5 Essential Cell Biology (© Garland Science 2010)

Table 15 -3 Essential Cell Biology (© Garland Science 2010)

Figure 15 -6 Essential Cell Biology (© Garland Science 2010)

Types of intracellular transport Figure 15 -5 Essential Cell Biology (© Garland Science 2010)

nuclear transport Figure 15 -7 Essential Cell Biology (© Garland Science 2010)

Figure 15 -8 Essential Cell Biology (© Garland Science 2010)

Figure 15 -9 Essential Cell Biology (© Garland Science 2010)

Energy for nuclear pore transport Figure 15 -10 Essential Cell Biology (© Garland Science 2010)

Types of intracellular transport Figure 15 -5 Essential Cell Biology (© Garland Science 2010)

Transport into mitochondria (unfolded proteins) Figure 15 -11 Essential Cell Biology (© Garland Science 2010)

Types of intracellular transport Figure 15 -5 Essential Cell Biology (© Garland Science 2010)

Endoplasmic reticulum Figure 15 -12 Essential Cell Biology (© Garland Science 2010)

Figure 15 -13 Essential Cell Biology (© Garland Science 2010)

(translation stalling) Figure 15 -14 Essential Cell Biology (© Garland Science 2010)

( -C-C- (cysteine) bridges formed in ER lumen) Figure 15 -15 Essential Cell Biology (© Garland Science 2010)

Insertion of (trans)membrane proteins Figure 15 -16 Essential Cell Biology (© Garland Science 2010)

Figure 15 -17 Essential Cell Biology (© Garland Science 2010)

protein secretion in bacteria

Figure 15 -23 Essential Cell Biology (© Garland Science 2010)

antibody processing

Types of intracellular transport Figure 15 -5 Essential Cell Biology (© Garland Science 2010)

endocytosis exocytosis (secretion) Figure 15 -18 Essential Cell Biology (© Garland Science 2010)

( ER ) ( cytoplasm ) Further sorting and modification in Golgi Figure 15 -26 Essential Cell Biology (© Garland Science 2010)

clathrin coated vesicles (to/from Golgi) Figure 15 -19 a Essential Cell Biology (© Garland Science 2010)

Figure 15 -19 b Essential Cell Biology (© Garland Science 2010)

clathrin coated vesicles Figure 15 -20 Essential Cell Biology (© Garland Science 2010)

membrane fusion does not occur spontaneously Figure 15 -21 Essential Cell Biology (© Garland

Figure 15 -22 Essential Cell Biology (© Garland Science 2010)

Figure 15 -27 Essential Cell Biology (© Garland Science 2010)

Figure 15 -28 Essential Cell Biology (© Garland Science 2010)

Endocytosis - Pinocytosis (liquids inc. fat) - phagocytosis Pinocytosis in macrophage: 25% own volume in 1 h = plasma membrane turnover in 30 min Figure 15 -32 Essential Cell Biology (© Garland Science 2010)

receptor mediated endocytosis (cholesterol example) ( LDL = low density lipoprotein ) Figure 15 -33 Essential Cell Biology (© Garland Science 2010)

Figure 15 -35 Essential Cell Biology (© Garland Science 2010)

Figure 15 -36 Essential Cell Biology (© Garland Science 2010)

Keywords Chapter 15: cytosol, nucleus, ER, golgi, mitochondria, lysosome, endosome, chloroplast, peroxisome signal sequence (receptor) transport types: nuclear, over membrane, vesicle nuclear transport cycle (nuclear pore, nuclear transport receptor, Ran(GTP)) transport mitochondria (receptor, translocase, unfolded proteins) transport ER (polyribosomes, SRP, translation stalling, signal peptidase) trans-membrane proteins (hydrophobic start/stop sequence) ER modifications: glycosylation (oligosaccharide protein transferase, dolichol (glucose, manose, N-acetylglucosamine)), disulphide bonds (e. g. antibody) endocytosis exocytosis cis/trans golgi network clathrin coated vesicles (cargo receptor, adaptin, clathrin, dynamin), rab-tethering protein, t/v snare proteins constitute/regulation secretion endocytosis, pinocytosis, phagocytosis cholesterol transport (LDL, endosome, lysosome) nuclease, protease, glycosidase, lipase, phosphatase (p. H in lysosome) autophagy

Glycosylation of secreted proteins occurs in ? (A) glycosomes (B) the nucleus (C) the golgi (D) the ER lumen Translation of a secreted protein continues ? (A) after SRP has delivered the ribosome to the translocation channel (B) after signal peptidase cleaves off the signal sequence (C) after the signal sequence finds the translocation channel (D) after the stop-transfer sequence is synthesised by the ribosome Pinocytosis is ? (A) uptake of liquids by phagocytosis (B) opposite of phagocytosis (C) uptake of liquids by endocytosis (D) exocytosis of liquid filled vesicles