Epithelialmesenchymal interaction EMI development of the kidney Epitheliomesenchymalis

  • Slides: 14
Download presentation
Epithelial-mesenchymal interaction (EMI); development of the kidney Epithelio-mesenchymalis kölcsönhatás: vese fejlődése

Epithelial-mesenchymal interaction (EMI); development of the kidney Epithelio-mesenchymalis kölcsönhatás: vese fejlődése

 • Epithelia: sheets or tubes of connected cells, originated from all germ layers

• Epithelia: sheets or tubes of connected cells, originated from all germ layers • Mesenchyme: loosely packed and unconnected cells, originated from mesoderm or neural crest

Time line. Development of the urinary system. Vizeletkiválasztó és nemi szervek fejlődése: részben közös

Time line. Development of the urinary system. Vizeletkiválasztó és nemi szervek fejlődése: részben közös telepekből indul mesoderma (középlemez, intermedier mesoderma) cloaca (endoderma) Urinary and genital organs - development common primordia mesoderm (intermediate mesoderm) cloaca (endoderm) partly from ectoderm (external genitalia)

21. és 25. nap, külső és belső glomerulusok days 21 and 25

21. és 25. nap, külső és belső glomerulusok days 21 and 25

5. hét, pronephros, mesonephros és metanephros 5 th week, pronephros, mesonephros and metanephros

5. hét, pronephros, mesonephros és metanephros 5 th week, pronephros, mesonephros and metanephros

Vese felszállása 1. Ascent of the kidneys 1.

Vese felszállása 1. Ascent of the kidneys 1.

Ureterbimbó belenő a metanephrogen szövetbe: kivezetőcsőrendszer kialakulása Ureteric bud: branching and growing

Ureterbimbó belenő a metanephrogen szövetbe: kivezetőcsőrendszer kialakulása Ureteric bud: branching and growing

Nephron kialakulása 1. Nephron development 1.

Nephron kialakulása 1. Nephron development 1.

Nephron fejlődése során expresszálódó molekulák (szövettenyészet) Molecules expressed during the development of the nephron

Nephron fejlődése során expresszálódó molekulák (szövettenyészet) Molecules expressed during the development of the nephron Proximalis kanyarulatos csatorna falának fejlődése Proximal convoluted tubule: development of the wall

Figure 15 -8 Positioning of ureteric bud formation is mediated by Ret signaling. Expression

Figure 15 -8 Positioning of ureteric bud formation is mediated by Ret signaling. Expression of Ret within the mesonephric duct epithelium is restricted to caudal portions of the duct by Bmp 4 released from cranial urogenital ridge mesenchyme. Mesenchymal expression of the Ret ligand, Gdnf, requires Pax 2 expression in the urogenital ridge but is restricted to the metanephric region by the cranial expression of Foxc 1, Foxc 2, and Slit 2 in the mesenchyme. The receptor for Slit 2 and Robo 2 is expressed in the urogenital ridge mesenchyme.

Figure 15 -9 Kidney patterning depends on interactions between the ureteric branches, condensing nephrogenic

Figure 15 -9 Kidney patterning depends on interactions between the ureteric branches, condensing nephrogenic mesenchyme, and interstitial stromal cells. Wt 1 expression is upregulated in the metanephric mesenchyme, making the mesenchyme receptive to ureteric bud induction. Wnt 4 released by the condensed mesenchyme induces continual ureteric bud branching and growth. Wnt 9 b, expressed by ureteric buds, promotes Wnt 4 expression within the condensing mesenchyme; Wnt 4 is necessary for maintaining the survival of this mesenchyme and for subsequent nephron differentiation. Stromal cells express Foxd 1, Pod 1, and Pdx 1 and the retinoic acid receptors, RARα and RARβ 2, necessary for balancing stromal and nephron progenitor specification and survival. The growth factors Fgf 2 and Bmp promote stromal cell differentiation at the expense of the nephron mesenchymal population. Moreover, retinoic acid signaling in stromal cells is required for maintaining Ret expression in the ureteric buds.

Vesefejlődés regulációja WT 1: transzkripciós faktor: epithel-mesenchyma interakció elősegítése GDNF, HGF: ureterbimbó növekedés serkentése

Vesefejlődés regulációja WT 1: transzkripciós faktor: epithel-mesenchyma interakció elősegítése GDNF, HGF: ureterbimbó növekedés serkentése RET, MET: GDNF-, HGF- receptorok az ureterbimbó sejtjein FGF 2, BMP 7: mesenchyma proliferáció serkentése, WT 1 expresszió fenntartása metanephrogen szövet mesenchymájának hámmá alakulása: laminin, IV. kollagén megjelenése, syndecan, E-cadherin szintézise szabályozó gének: PAX 2, WNT 4 Regulation of the development of the kidney WT 1: transcription factor: role in the epithelial-mesenchymal interaction GDNF, HGF: stimulation of the enlargement of the ureteric bud RET, MET: GDNF-, HGF- receptors on the cells of the ureteric bud FGF 2, BMP 7: inducing the mesenchyme proliferation, and the WT 1 expression epithelial transformation of the metanephrogen tissue mesenchyme: synthesis of laminin, collagen type IV. , syndecan, E-cadherin regulatory genes: PAX 2, WNT 4

Figure 16 -3 Molecular basis for the early formation of the metanephros and ureter.

Figure 16 -3 Molecular basis for the early formation of the metanephros and ureter. A, Early induction of metanephros. B, Branching of ureteric bud. C, Early tubule formation. FGF, fibroblast growth factor; GDNF, glial cell line-derived neurotrophic factor; LIF, leukemia inhibitory factor.