epithelial cells nonepithelial cells tight junctions actin adhesion

epithelial cells non-epithelial cells tight junctions actin adhesion belt (CADHERINS) CELL ADHESION desmosomes (CADHERINS) Gap junctions (connexins) CELL MATRIX ADHESION hemidesm. (integrins) focal contacts (integrins) basal lamina CADHERINS IG-LIKE CAMS INTEGRINS SELECTINS integrins NO attachment plague Golan H. , 2006 Non-JUNCTIONAL CONTACTS JUNCTIONAL ADHESION MECHANISMS

מולקולות המשתייכות לקבוצה Ig superfamily Cadherins Integrins Netrins Semaphorines Laminims Collagen Tenascins Receptor tyrosin phosphatase& kinase Golan H. , 2006

CELL-CELL adhesion Cadh. repeats CELL ADHESION MOLECULES Cadherins Ca 2+ -dep. homophilic adhesion functional unit = dimer Ig and Fn III repeats Immunoglobin superfamily (CAMs) homophilic or heterophilic lectin repeats Selectins heterophilic P selectin + counter-receptor PSGL-1, glycosylated I-CAM Integrins heterodimers, heterophilic bind to ECM, Ig-CAMs, cadherins adhesion, polarity, migration Golan H. , 2006 Hynes: Ti. CB: 9: M 33 1999

Some adhesion molecules involved in axon growth Cell adhesion molecules (CAMs) Name Similar/identical molecules Comments N-cadherin (mouse) N-Cal. CAM (chick) A-CAM (chick) BSP-2 NCAM LI (mouse) TAG-1 (rat) NILE* (rat) G 4, Ng-CAM, 809 (chick) Nr-CAM, neurofascin Neuroglian (insects) Axonin-1, F 11 Fasciclins Golan H. , 2006 Calcium-dependent Immunoglobulin superfamily Insects. Fasciclin II is the orthologue

Some adhesion molecules involved in axon growth Substrate adhesion molecules (SAMs) Name laminin Fibronectin Tenascin Similar/identical molecules Comments Binds to Integrin Cytotactin, J 1, myotendinous antigen Collagen Golan H. , 2006

CAM -Cell Adhesion Molecule In neurons: N-CAM Ig superfamily שייך ל (At least one Ig-like domain with type II fibronectin domain) Known ~20 different types Formed by alternative splicing Ca independent פוליפפטיד גדול לרוב אזור טרנסממברנלי וזנב ציטוסולי קיימת גם צורה המופרשת מהתא Golan H. , 2006 לרוב קישור הומופילי

N-CAM in neurogenesis and synaptic plasticity Golan H. , 2006

PSA-NCAM - red PSA-NCAM Brd. U Golan(SVZ) H. , 2006 and olfactory bulb of the adult rat PSA-NCAM in the subventricular zone

PSA-NCAM in the cerebellum of peri-puberal rabbit Golan H. , 2006

Fas II mechanism of action (K channel mutant (EAG Golan H. , 2006 (c. AMP mutant (dunce

Intra cellular vinculin a-actinin talin signalling molecules fibronectin laminin Integrin a 5 b 1 = fibronectin receptor Golan H. , 2006 Integrin a 6 b 1 = laminin receptor

Golan H. , 2006

Neuroligin-1 Golan H. , 2006 PDZ binding domain

Neuroligin-1 Neurologin interacts mainly with the 3 rd PDZ domain in the PSD 95 Interact also with: SAP 102, PSD-93, S-SCAM, Magi 1 and 3, Shank 1 and 3, Pick 1, GOPC, SPAR, PSD 953 PDZ domains, SH 3 domain, guanilate kinase domain Golan H. , 2006

Neurexin/s Neurologin binding to its receptors: a or b neurexin depends on alternative splicing of neuroligin. Neuroligin signaling depends on: Protein interaction in the PSD Neoroligin splice variant Ca Protein oligomerization/dimerization Neurexin type Golan H. , 2006 Neurexin interaction in the presynaptic terminal

Neuroligin/Neurexin clustering in the synapse Involvement in development: Neurologin 1 - Expression increased postnatally (P 1 -P 8) Neurexin – expression from. Golan E 10 H. , 2006

Neuroligin/Neurexin and synaptic strength Golan H. , 2006

Intra cellular vinculin a-actinin talin signalling molecules fibronectin laminin Integrin a 5 b 1 = fibronectin receptor Golan H. , 2006 Integrin a 6 b 1 = laminin receptor

Sakisaka, T. et al. J Cell Sci 2005; 118: 5407 -5410 Golan H. , 2006

Golan H. , 2006

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Cell adhesion molecules involved in axonal guidance and path finding Robo - slit Fas II Golan H. , 2006

Eph and Eph receptor Golan H. , 2006